JMY antibody (N-Term)
-
- Target See all JMY Antibodies
- JMY (Junction Mediating and Regulatory Protein, P53 Cofactor (JMY))
-
Binding Specificity
- N-Term
-
Reactivity
- Human, Mouse, Rat, Cow, Guinea Pig
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This JMY antibody is un-conjugated
-
Application
- Western Blotting (WB)
- Sequence
- VFIVAWNEIE GKFAITCHNR TAQRQRSGSR EQAGARGGAE AGGAASDGSR
- Predicted Reactivity
- Cow: 86%, Guinea Pig: 93%, Human: 100%, Mouse: 100%, Rat: 100%
- Characteristics
- This is a rabbit polyclonal antibody against JMY. It was validated on Western Blot.
- Purification
- Affinity Purified
- Top Product
- Discover our top product JMY Primary Antibody
-
-
- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
-
Antigen size: 712 AA
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
-
- Target
- JMY (Junction Mediating and Regulatory Protein, P53 Cofactor (JMY))
- Alternative Name
- JMY (JMY Products)
- Synonyms
- WHDC1L3 antibody, junction mediating and regulatory protein, p53 cofactor antibody, junction-mediating and regulatory protein antibody, JMY antibody, jmy antibody, Jmy antibody
- Background
-
JMY acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, JMY acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. JMY increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, JMY acts as a nucleation-promoting factor for both branched and unbranched actin filaments. JMY activates the Arp2/3 complex to induce branched actin filament networks. JMY also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. JMY contributes to cell motility by controlling actin dynamics. JMY may promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location.
Alias Symbols: FLJ37870, MGC163496, WHDC1L3
Protein Interaction Partner: CCDC136, UBC, EP300, MDM2, E2F4, E2F3, E2F2, E2F1,
Protein Size: 712 - Molecular Weight
- 82 kDa
- Gene ID
- 133746
- NCBI Accession
- NM_152405, NP_689618
- UniProt
- Q8N9B5
- Pathways
- Regulation of Actin Filament Polymerization
-