AGO4 antibody (AA 401-500) (Alexa Fluor 594)

Catalog No. ABIN2809910

Product Details anti-AGO4 Antibody (Alexa Fluor 594)

Target: AGO4 (EIF2C4) (Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4))

Binding Specificity: AA 401-500

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGO4 antibody is conjugated to Alexa Fluor 594

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Argonaute 4/eIF2C4

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for AGO4

Target: AGO4 (EIF2C4) (Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4))

Alternative Name: Argonaute 4/e2C4 (EIF2C4 Products)

Synonyms: EIF2C4 antibody, ago4 antibody, Argonaute4 antibody, argonaute-4 antibody, eif2c4 antibody, wu:fd14f04 antibody, ARGONAUTE 4 antibody, OCP11 antibody, OVEREXPRESSOR OF CATIONIC PEROXIDASE 11 antibody, T20P8.9 antibody, T20P8_9 antibody, Eif2c4 antibody, 5730550L01Rik antibody, AI481660 antibody, argonaute 4, RISC catalytic component antibody, argonaute 1, RISC catalytic component antibody, argonaute RISC catalytic component 4 antibody, Argonaute family protein antibody, argonaute 4, RISC catalytic component L homeolog antibody, argonaute RISC catalytic subunit 4 antibody, AGO4 antibody, ago1 antibody, ago4 antibody, Ago4 antibody, ago4.L antibody

Background:

Synonyms: 5730550L01Rik, Ago 4, Ago4, AGO4_HUMAN, Argonaute4, E 2C 4, e-2C 4, e2C 4, E2c4, eukaryotic translation initiation factor 2C 4, eukaryotic translation initiation factor 2C, hAgo4, KIAA1567, Piwi/Argonaute family protein me2C4, Protein argonaute-4.

Background: Eukaryotic translation initiation factor 2C (eIF2C) proteins (argonaute family) influence RNA interference (RNAi) as components of the RNA-inducible silencing complex (RISC) or microRNA (miRNA)-containing ribonucleoprotein particle (miRNP). Small RNAs, including small interfering RNAs (siRNAs) and miRNAs, can silence target genes through mechanisms that utilize RISC or miRNP particles. eIF2C1 (argonaute 1, AGO1, eIF2C, GERP95, Q99) and Dicer1 play a coordinated role in siRNA-mediated gene silencing. eIF2C2 (Slicer, argonaute 2, AGO2, Q10) is a RISC component that can concentrate in cytoplasmic processing bodies (P-bodies) and catalyze mRNA cleavage. Mammalian P-bodies contain mRNAs and have an association with miRNA-induced translational silencing and siRNA-induced mRNA degradation. Additional eIF2C proteins include eIF2C3 (argonaute 3, AGO3), eIF2C4 (argonaute 4, AGO4) and meIF2c5 (mouse argonaute 5).

Gene ID: 192670

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Cellular Glucan Metabolic Process