EPH Receptor A4 antibody (EPHA4) (AA 40-70)

Details for Product anti-EPHA4 Antibody No. ABIN2841709, Supplier: Log in to see
Antigen
  • HEK8
  • SEK
  • TYRO1
  • RGD1560587
  • ephe
  • EPHA4
  • Ci-Eph1
  • sek
  • hek8
  • rtk1
  • tyr01
  • 2900005C20Rik
  • AI385584
  • Cek8
  • Hek8
  • Sek
  • Sek1
  • Tyro1
  • rb
  • XEpha4
  • epha4
  • pag
  • sek-1
  • EphA4
  • apha3
  • ek2
  • epha3
  • sb:cb246
  • zek2
  • zgc:109882
  • EPH receptor A4
  • Eph receptor A4
  • ephrin receptor epsilon
  • EPH receptor A4 L homeolog
  • eph receptor A4a
  • EPHA4
  • Epha4
  • ephe
  • epha4
  • epha4.L
  • epha4a
Alternatives
anti-Mouse (Murine) EPH Receptor A4 antibody for Western Blotting
Epitope
AA 40-70, N-Term
30
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20
15
13
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11
10
7
5
4
4
4
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
156
72
37
10
10
10
7
7
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6
4
4
2
2
2
2
2
1
1
1
Host
Rabbit
149
23
17
1
Clonality (Clone)
Polyclonal ()
Conjugate
This EPH Receptor A4 antibody is un-conjugated
9
8
8
6
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6
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
133
84
84
51
19
17
13
7
4
2
1
1
Options
Supplier
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Immunogen This EphA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-70 AA from the N-terminal region of human EphA4.
Clone RB1587
Isotype Ig
Predicted Reactivity Chicken,Mouse (Murine),Xenopus laevis
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name EphA4 (EPHA4 Antibody Abstract)
Background Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

Synonyms: Ephrin type-A receptor 4 , Tyrosine-protein kinase receptor SEK, Receptor protein-tyrosine kinase HEK8
Molecular Weight 109859 Da
Gene ID 2043
UniProt P54764
Research Area Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Metabolism, Cell Structure
Pathways RTK Signaling
Application Notes Suggested dilutions:
WB = 1:1000, IF = 1:100, FACS = 1:10-50, IHC (p) = 1:50-100
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 1.11 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-EPH Receptor A4 (EPHA4) (AA 40-70), (N-Term) antibody (ABIN2841709) Western blot analysis of anti-EphA4 N-term Pab (ABIN391889) in HeLa cell lysate. EphA...
Western Blotting (WB) image for anti-EPH Receptor A4 (EPHA4) (AA 40-70), (N-Term) antibody (ABIN2841709) Western blot analysis of EphA4 (arrow) using EphA4 Antibody (N-term) (ABIN391889). 29...
Immunofluorescence (IF) image for anti-EPH Receptor A4 (EPHA4) (AA 40-70), (N-Term) antibody (ABIN2841709) Methanol/Acetone fixed human stem cell is used in IF to detect Eph4A (blue) and endot...
Background publications Prevost, Woulfe, Tanaka, Brass: "Interactions between Eph kinases and ephrins provide a mechanism to support platelet aggregation once cell-to-cell contact has occurred." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 14, pp. 9219-24, 2002 (PubMed).

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