IL-1 beta antibody (Biotin)

Catalog No. ABIN288401

The Goat Polyclonal anti-IL-1 beta antibody is suitable to detect IL-1 beta in samples from Human. It has been validated for WB, IHC and ELISA.

Quick Overview for IL-1 beta antibody (Biotin) (ABIN288401)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IL-1 beta antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-IL-1 beta Antibody (Biotin)

Sterility: Filtered

Immunogen: IL1 b antibody (biotin) was raised in goat using E. Coli derived recombinant human IL1?/IL1 2 as the immunogen.

Isotype: IgG

Application Details

Application Notes: ELISA: 2-8 µg/mL, IHC: 515 µg/mL, WB: 0.1 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute to a concentration of 50 µg/mL with 1 mL of sterile 20 mM Trizma Base, 150 mM NaCl, pH 7.3 containing 0.1 % BSA.

Concentration: Lot specific

Buffer: Lyophilized from sterile filtered PBS solution with BSA.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: IL1b

Background: IL1 is a name that designates two pleiotropic cytokines, IL1 alpha (IL1F1) and IL1Beta (IL1F2), which are the products of distinct genes. IL1 alpha and IL1Beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL1 alpha and IL1Beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL1 RI binds directly to IL1 alpha or IL1Beta and then associates with IL1 R accessory protein (IL1 R3/IL1 R AcP) to form a highaffinity receptor complex that is competent for signal transduction. IL1 RII has high affinity for IL1Beta but functions as a decoy receptor and negative regulator of IL1Beta activity.

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome