TEK antibody

Catalog No. ABIN5676011

The Rabbit Polyclonal anti-TEK antibody is suitable to detect TEK in samples from Human. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for TEK antibody (ABIN5676011)

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TEK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-TEK Antibody

Specificity: This antisera is monospecific to B-2 Microglobulin. Virtually no crossreactivity to other human proteins

Cross-Reactivity (Details): This human TIE2 sequence is 100 % conserved in rat, mice, and 94 % bovine Tie-2. The peptide is specific for Tie-2 as no significant sequence homology is seem with Tie-1.

Purification: Affinity chromatography purified

Immunogen: TIE2 antibody was raised in rabbit using an 18 amino acid peptide from human TIE2 as the immunogen.

Isotype: IgG

Application Details

Application Notes: ELISA: 1:10,000-1:100,000, HC: 2-20 µg/mL, IF: 2-20 µg/mL, WB: 1-10 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from 100 mM Tris, pH 7.5, with 0.2 % BSA, and 0.05 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

Target Details for TEK

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Alternative Name: TIE2

Background: The family of receptor tyrosine kinases TIE1 and TIE 2 or Tek has been identified in vascular endothelium and hematopoietic cells. Ties may represent the earliest endothelial cell lineage marker and may regulate the endothelial cell proliferation, differentiation, and proper patterning during vasculogenesis.

Pathways: RTK Signaling, Growth Factor Binding