MICA/B antibody

Catalog No. ABIN291612

The Mouse Monoclonal anti-MICA/B antibody (ABIN291612) specifically detects MICA/B in FACS, IHC (fro), IP and IHC. The antibody is reactive with Human samples.

Quick Overview for MICA/B antibody (ABIN291612)

Target: MICA/B (MICA/MICB) (MHC Class I Polypeptide-Related Sequence A/B (MICA/MICB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MICA/B antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Product Details anti-MICA/B Antibody

Specificity: Specific for nonclassical MHC class I chain A (MICA) and nonclassical MHC class I chain MICB (MICB). MICA and MICB are stress inducible antigens, which are closely related and appear functionally indistinguishable. MICA and MICB are ligands for NKG2D, an activating receptor on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. MICA is principally expressed on intestinal epithelium, and several epithelial tumors. Expression may be induced to high surface levels by heat shock, oxidative stress, and virus infection. Clone 6D4 is reported to inhibit the cytotoxicity of NK cells stimulated by IFN alpha-treated dendritic cells. XXX recommend the use of MCA2304EL for such studies.

Purification: Protein G purified

Immunogen: MICA transfected C1R cells.

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo, IHC, IHC-Fr (1:100), IP

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Method sheets are available on request. Membrane permeabilization is required for this application.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for MICA/B

Target: MICA/B (MICA/MICB) (MHC Class I Polypeptide-Related Sequence A/B (MICA/MICB))