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MICA/B antibody

MICA/MICB Reactivity: Human FACS, IHC, IP Host: Mouse Monoclonal 6D4 unconjugated
Catalog No. ABIN7013904
  • Target See all MICA/B (MICA/MICB) products
    MICA/B (MICA/MICB) (MHC Class I Polypeptide-Related Sequence A/B (MICA/MICB))
    Reactivity
    Human
    Host
    • 15
    • 7
    Mouse
    Clonality
    • 16
    • 6
    Monoclonal
    Conjugate
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This MICA/B antibody is un-conjugated
    Application
    • 13
    • 13
    • 6
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)
    Specificity
    The mouse monoclonal antibody 6D4 recognizes a common extracellular epitope on MICA and MICB glycoproteins, transmembrane ligands of CD314 (NKG2D), and is able to block CD314-mediated activation of NK cells and cytotoxic T cells.
    Purification
    Purified by protein-A affinity chromatography.
    Immunogen
    Transfected C1R cells expressing MICA
    Clone
    6D4
    Isotype
    IgG2a
  • Application Notes
    Flow cytometry: Recommended dilution: 1-4 μg/mL.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Target
    MICA/B (MICA/MICB) (MHC Class I Polypeptide-Related Sequence A/B (MICA/MICB))
    Alternative Name
    MICA/MICB (MICA/MICB Products)
    Background
    MICA and MICB glycoproteins are members of MHC class I family, closely linked to HLA-B. However, unlike HLA molecules, MICA and MICB are not associated with beta2 microglobulin and are conformationally stable in the absence of conventional MHC class I peptide ligands. Both proteins are stress-induced antigens expressed mainly in gastrointestinal epithelium, where they are recognized by V-delta1 subset of gamma/delta T cells, and also on diverse epithelial tumor cells. Binding of MICA/MICB receptor, the NKG2D, leads to cytolytic response of NK cells, Tc cells, and gamma/delta T cells. Alternative splicing results in multiple isoforms, and some of them have been associated with susceptibility to psoriasis and psoriatic arthritis. Shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma.
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