Mouse anti-Cow IgL Antibody
-
- Target See all IgL products
- IgL
- Reactivity
- Cow
-
Host
- Mouse
-
Clonality
- Monoclonal
- Application
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- Reacts with bovine IgG (light chains). IgG light chains (kappa and lambda types) consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The antibody recognizes the light chain regardless it is free or attached to the heavy chain.
- Purification
- Protein A purified
- Immunogen
- Fraction of Bovine immunoglobulin.
- Clone
- IVA285-1
- Isotype
- IgG
-
- Application Notes
-
Approved: Flo, IHC, IHC-Fr, WB (1:50, 5 - 1 μg/mL)
Usage: Western Blotting: Recommended dilution - 0,5-1 μg/mL, overnight in 4 degrees C. Positive control and sample preparation: BS (bovine serum), dilution 1:50 in Laemmli reducing buffer, boiled in water bath for 3 min. Application notes:. Strongly reacts with - Comment
-
Target Species of Antibody: Bovine
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, 15 mM sodium azide, approx, pH 7.4.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid freeze thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
-
- Target
- IgL
- Alternative Name
- Ig Light Chain (IgL Products)
-