MBD3 antibody (AA 215-230)

Catalog No. ABIN294235

This Mouse Monoclonal antibody specifically detects MBD3 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Dog, Cow, Horse, Pig, Bat, Chicken and Xenopus laevis.

Quick Overview for MBD3 antibody (AA 215-230) (ABIN294235)

Target: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Reactivity: Human, Dog, Cow, Horse, Pig, Bat, Chicken, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This MBD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 106B691

Product Details anti-MBD3 Antibody

Binding Specificity: AA 215-230

Specificity: The theoretical molecular weight of human MBD2 (411 amino acids) is 45 kD and human MBD3 (291 amino acids) is 33 kD. Based on sequence identity, this will detect human, mouse, and rat MBD3 (100 % ), and human and mouse MBD2 (93 %).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus (100%) Marmoset, Mouse, Rat, Hamster, Rabbit, Stickleback, Pufferfish, Zebrafish (94%) Salmon (88%).

Purification: Protein G purified

Immunogen: Amino acids 215-230 (CKAFMVTDEDIRKQEE) of human MBD3 protein were used as the immunogen for this antibody. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus (100%), Marmoset, Mouse, Rat, Hamster, Rabbit, Stickleback, Pufferfish, Zebrafish (94%), Salmon (88%).

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide. Sourced in TCS.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for MBD3

Target: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Alternative Name: MBD3

Background: Name/Gene ID: MBD3

Synonyms: MBD3

Gene ID: 53615

UniProt: O95983

Pathways: Chromatin Binding