MBD3 antibody (C-Term)

Catalog No. ABIN6263149

Product Details anti-MBD3 Antibody

Target: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBD3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: MBD3 Antibody detects endogenous levels of total MBD3.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human MBD3, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for MBD3

Target: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Alternative Name: MBD3 (MBD3 Products)

Background:

Description: Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (PubMed:12124384). Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed:24307175). Recruits histone deacetylases and DNA methyltransferases.

Gene: MBD3

Molecular Weight: 33kDa

Gene ID: 53615

UniProt: O95983

Pathways: Chromatin Binding