SMAD6 antibody (AA 85-99)

Catalog No. ABIN295868

The Rabbit Polyclonal anti-SMAD6 antibody is suitable to detect SMAD6 in samples from Human and Pig. It has been validated for WB, IHC and IHC (p).

Quick Overview for SMAD6 antibody (AA 85-99) (ABIN295868)

Target: SMAD6 (SMAD, Mothers Against DPP Homolog 6 (SMAD6))

Reactivity: Human, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SMAD6 Antibody

Binding Specificity: AA 85-99

Specificity: A 52 kD band is observed.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Pig (100%) Dog, Bovine, Opossum (93%) Gibbon, Marmoset, Rat (87%) Elephant (80%).

Purification: Protein G purified

Immunogen: This antibody was developed against synthetic peptides corresponding to amino acids 85-99 (RAGGPPRPMSEPGAG)and amino acids 372-388 (NLEQRSESVRRTRSKIG)of human SMAD6 (GenBank Accession No. AAH12986.1). These peptide sequences are specific to SMAD6, and have high homology to SMAD6in multiple species. Percent identity by BLAST analysis: Human, Pig (100%), Dog, Bovine, Opossum (93%), Gibbon, Marmoset, Rat (87%), Elephant (80%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % BSA, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for SMAD6

Target: SMAD6 (SMAD, Mothers Against DPP Homolog 6 (SMAD6))

Alternative Name: SMAD6

Background: Name/Gene ID: SMAD6

Synonyms: SMAD6, AOVD2, HSMAD6, HsT17432, SMAD 6, MADH7, Mothers against DPP homolog 6, SMAD family member 6, MAD homolog 6, MADH6

Gene ID: 4091

UniProt: O43541

Pathways: Chromatin Binding