SMAD6 antibody (N-Term)

Catalog No. ABIN2779804

The Rabbit Polyclonal anti-SMAD6 antibody is suitable to detect SMAD6 in samples from Human, Mouse, Rat, Cow, Pig, Dog, Guinea Pig, Horse and Zebrafish (Danio rerio). It has been validated for WB and IHC.

Quick Overview for SMAD6 antibody (N-Term) (ABIN2779804)

Target: SMAD6 (SMAD, Mothers Against DPP Homolog 6 (SMAD6))

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SMAD6 Antibody

Binding Specificity: N-Term

Purpose: SMAD6 Antibody - N-terminal region

Sequence: APRDASDPLA GAALEPAGGG RSREARSRLL LLEQELKTVT YSLLKR

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 91%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against SMAD6. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SMAD6

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8111902

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for SMAD6

Target: SMAD6 (SMAD, Mothers Against DPP Homolog 6 (SMAD6))

Alternative Name: SMAD6

Background:

Background Information: SMAD6 (mothers against decapentaplegic homolog 6) is an antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members, has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD.

Gene Name: SMAD family member 6

Alternative Symbols: AOVD2, MADH6, MADH7, HsT17432

Protein Name: Mothers against decapentaplegic homolog 6

Molecular Weight: 53kDa

Gene ID: 4091

NCBI Accession: NP_005576

UniProt: O43541

Pathways: Chromatin Binding