AGRP antibody (AA 119-132)

Catalog No. ABIN296668

Product Details anti-AGRP Antibody

Target: AGRP (Agouti Related Protein Homolog (Mouse) (AGRP))

Binding Specificity: AA 119-132

Reactivity: Human, Bat, Horse, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This AGRP antibody is un-conjugated

Application: ELISA

Specificity: Human AGRP. This antibody is expected to recognise both reported isoforms (as represented by NP_001129, NP_015531)

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Bat, Horse (100%) Dog, Pig (93%) Mouse, Hamster, Elephant, Zebu, Bovine (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide CRKLGTAMNPCSRT from the C-terminus of human AGRP (NP_001129.1, NP_015531.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Bat, Horse (100%), Dog, Pig (93%), Mouse, Hamster, Elephant, Zebu, Bovine (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:32000)

Usage: Preliminary experiments gave bands at approx 45  kDa and 24  kDa in Human Brain lysates after 0.1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 14.4  kDa according to NP_001129.1and NP_015531.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for AGRP

Target: AGRP (Agouti Related Protein Homolog (Mouse) (AGRP))

Alternative Name: AGRP (AGRP Products)

Synonyms: AGRT antibody, ART antibody, ASIP2 antibody, Agrt antibody, Art antibody, agouti related neuropeptide antibody, agouti-related protein pseudogene antibody, AGRP antibody, Agrp antibody, LOC100352591 antibody

Background: Name/Gene ID: AGRP

Synonyms: AGRP, Agouti (mouse) related protein, Agouti-related protein, Agouti-related transcript, AGRT, ASIP2

Gene ID: 181

NCBI Accession: NP_001129, NP_015531

UniProt: O00253

Pathways: Feeding Behaviour, Photoperiodism