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Integrin beta 4 antibody (Extracellular Domain)

ITGB4 Reactivity: Human WB, FACS, ELISA, ICC Host: Sheep Polyclonal unconjugated

Catalog No. ABIN298749

Datasheet as PDF

Target See all Integrin beta 4 (ITGB4) Antibodies

Integrin beta 4 (ITGB4)
Binding Specificity
15

10

6

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Extracellular Domain
Reactivity
75

24

23

3

3
Human
Host
54

33

7

1
Sheep
Clonality
48

47
Polyclonal
Conjugate
47

8

7

3

3

3

3

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1
This Integrin beta 4 antibody is un-conjugated
Application
46

39

28

27

18

13

13

13

13

9

7

7

6

2

2

1

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunocytochemistry (ICC)

Specificity

Selected for its ability to recognize human Integrin B4 in the applications listed. In direct ELISAs and Western Blots, this antibody shows ~25 % cross-reactivity with rmIntegrin B4, and less than 1 % cross-reactivity with rhIntegrin B2, rhIntegrin B3, and rhIntegrin B5.

Purification

Affinity purified

Immunogen

CHO cell-derived rhIntegrin B4 extracellular domain.

Isotype

IgG

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ITGB4 Reactivity: Human WB, IHC, FACS, ELISA, ICC, Neut Host: Mouse Monoclonal 5G3G5 unconjugated

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ITGB4 Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

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ITGB4 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

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ITGB4 Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated

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ITGB4 Reactivity: Human WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated

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ITGB4 Reactivity: Human WB, ELISA Host: Mouse Polyclonal unconjugated

anti-Integrin beta 4 (ITGB4) antibody
ITGB4 Reactivity: Human WB Host: Rabbit Monoclonal unconjugated

anti-Integrin beta 4 (ITGB4) (AA 1098-1356) antibody
ITGB4 Reactivity: Mouse WB, IHC, ICC Host: Rabbit Polyclonal unconjugated

anti-Integrin beta 4 (ITGB4) (AA 28-266) antibody
ITGB4 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal unconjugated

Application Notes

Approved: ELISA (0.5 - 1 μg/mL), Flo (50 μg/mL), ICC, WB

Usage: Suitable for use in ELISA, Western Blot, Immunocytochemistry and Flow Cytometry. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: 0.5-1.0 μg/mL. The detection limit is ~0.3 ng/well. Flow cytometry: 50 μg/mL. Add 10 μL to 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL.

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

0.5 mL sterile PBS

Concentration

Lot specific

Buffer

Lyophilized from PBS with 5 % trehalose

Handling Advice

Avoid repeated freezing and thawing.

Storage

4 °C,-20 °C

Storage Comment

4°C or -20°C, Avoid freeze-thaw cycles.
Target

Integrin beta 4 (ITGB4)

Alternative Name

ITGB4 / Integrin Beta 4 (ITGB4 Products)

Synonyms

AA407042 antibody, C230078O20 antibody, CD104 antibody, integrin subunit beta 4 antibody, integrin beta 4 antibody, ITGB4 antibody, Itgb4 antibody

Background

Name/Gene ID: ITGB4
Family: Integrin

Synonyms: ITGB4, CD104, Integrin beta-4, Integrin Beta 4, gp150, CD104 antigen, Integrin beta-4 subunit, Integrin, beta 4

Gene ID

3691

UniProt

P16144

Pathways

Integrin Complex