Mouse anti-Human IgG (Fc Region) Antibody (HRP)

Details for Product No. ABIN302063, Supplier: Log in to see
Antigen
Epitope
Fc Region
4680
2536
1880
1066
1006
860
300
294
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
Reactivity
Human
2848
2739
2297
1675
1433
788
613
442
380
379
358
339
291
290
208
138
135
122
41
29
27
25
12
11
9
8
6
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
Host
Mouse
6708
4832
1795
890
609
388
91
55
52
21
8
5
5
5
2
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
HRP
2234
1872
1744
1356
767
682
519
297
264
223
222
200
156
138
128
110
79
75
69
55
51
48
46
43
40
38
34
34
32
29
27
27
27
27
27
27
27
27
22
22
19
19
19
19
15
15
15
15
15
15
14
12
12
10
10
9
9
9
9
9
9
9
8
8
8
8
8
6
6
6
6
6
5
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
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Supplier Product No.
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Immunogen Fusion protein of human IgG Fc fragment.
Clone EM-07
Isotype IgG1
Specificity The antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.
Characteristics The purified antibody is conjugated with Horseradish Peroxidase (HRP) of high specific activity and RZ=3.
Background Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75 % of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.
Molecular Weight 150 kDa
Research Area Immunology, Secondary Antibodies
Application Notes The peroxidase conjugate is designed for ELISA and Western Blotting. Suggested working dilution for ELISA is 0.1 - 0.5 µg/mL. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 0.01 % (w/v) thimerosal and 1 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Preservative Thimerosal (Merthiolate)
Precaution of Use Biohazard Informations: This product contains thimerosal which is hazardous.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8 °C. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
Supplier Images
Western Blotting (WB) image for Mouse anti-Human IgG (Fc Region) antibody (HRP) (ABIN302063) IgG κ light chain (1), IgG λ light chain (2) and IgG Fc fragment (3) purifi...
Background publications Brinkmann, Heusser: "T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors." in: Cellular immunology, Vol. 152, Issue 2, pp. 323-32, 1994 (PubMed).

Balogh, Merétey, Falus, Bozsóky: "Serological abnormalities in juvenile chronic arthritis: a review of 46 cases." in: Annals of the rheumatic diseases, Vol. 39, Issue 2, pp. 129-34, 1980 (PubMed).

Franklin: "Structure and function of immunoglobulins." in: Acta endocrinologica. Supplementum, Vol. 194, pp. 77-95, 1975 (PubMed).

Fuller, Keyser: "Serum immunoglobulins after surgical operation." in: Clinical chemistry, Vol. 21, Issue 6, pp. 667-71, 1975 (PubMed).