GFP antibody

Antigen: GFP

Clonality: Polyclonal

Host: Rabbit

Reactivity: All Species

Application: Immunoprecipitation (IP), Western Blotting (WB), Immunocytochemistry (ICC)

Catalog no.: ABIN302066

Additional Information

Immunogen: EGFP, a native full-length protein

Format: Purified

Description: Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscentjellyfish Aquorea victoria, emiting green light ( λ =509 nm) when excited (excitation by Blue or UV light, absorption peak λ =395 nm). GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized inliving cells. Light-stimulated GFP fluorescence is species-independent and a fluorescencehas been reported from many different types of GFP-expressing hosts, including microbes,invertebrates, vertebrates and plants. No exogenous substrates and cofactors are requiredfor the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment fromnatural amino acids present in the nascent protein.GFP fluorescence is stable under fixation conditions and suitable for a variety ofapplications. GFP is widely used as a reporter (tag) for gene expression, enablingresearchers to visualize and localize GFP-tagged proteins within living cells without anyfurther staining. Other applications of GFP include measurement of distance betweenproteins through fluorescence energy transfer (FRET) protocols. To increase a fluorescenceintensity of GFP, chomophore mutations have been created. The EnhancedGFP has afluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has producedalso many mutants (e.g. Yellow Fluorescent Protein, Cyan Fluorescent Protein) with waryingspectral properties. Antibodies raised against full-length GFP variants should also detectother variants of the protein.

Specificity: The polyclonal antibody recognizes GFP, EGFP, EYFP fusion proteins in all species.

Application Details

Application Notes: Immunoprecipitation - Recommended dilution: 10-20 µg/sample. Western Blotting: - Recommended dilution: 0.5-1. 5 µg/ml. Positive control: transfected cells. Negative control: non-transfected cells. Immunocytochemistry - Recommended dilution: Purified Antibody: 1-3 µg/ml. Positive control: transfected cells. Negative control: non-transfected cells

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from rabbit serum by protein-A afinity chromatography.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Tags/Labels

Restrictions: For Research Use only

Images

Confocal microscopy

Publications

Publications: Porrero, Rubio-Garrido, Avendauno et al.: "Mapping of fluorescent protein-expressing neurons and axon pathways in adult and developing Thy1-eYFP-H transgenic mice." in: Brain research, 2010 (PubMed).