The mouse monoclonal antibody C5 recognizes human CRP, a 117 kDa plasma acute phase protein, it recognizes antigen both in the presence and in the absence (samples containing EDTA) of Ca2+.
CRP
Reactivity: Rat
WB, IHC, ICC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: The antibody C5 has been tested as the capture antibody in a sandwich ELISA for analysis of human C-Reactive Protein in combination with the antibody C7 (cat. no. 1B-484-C100).
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Do not freeze.
Storage
4 °C
Storage Comment
Store at 2-8°C. Do not freeze.
Krämer, Kess, Kremmer, Schulte-Hostede: "Multi-parameter determination of TNF?, PCT and CRP for point-of-care testing." in: The Analyst, Vol. 136, Issue 4, pp. 692-5, (2011) (PubMed).
C-reactive protein,The C-reactive protein (CRP) is a cyclic pentameric pentraxin family acute phase protein composed of five identical noncovalently bound nonglycosylated subunits (each subunit 24 kDa, physiologic CRP molecule 117,5 kDa). CRP is produced by the liver and its plasma levels rise dramatically during inflammatory processes occuring in the body. CRP is an initiator of classical complement cascade, binds to several nuclear components (chromatin, histones, etc.) and is also believed to play an important role in innate immunity. Patients with elevated basal levels of CRP are at increased risk for hypertension and cardiovascular disease.