Growth Factor Receptor-Bound Protein 2 (GRB2) antibody

Antigen: Growth Factor Receptor-Bound Protein 2 (GRB2)

Synonyms: ASH, Grb3-3, MST084, NCKAP2, MSTP084, EGFRBP-GRB2, Ash, AA408164, MGC108668, zgc:73077, wu:fb18e12, wu:fb34e06, GRB2, MGC127576, DKFZp459P153, grb2, MGC64880, BB233594, 8430435N19Rik, MGC112733, GRAP, MGC166297, MGC110202, zgc:110202, MGC109892, si:dkeyp-9d4.1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN302091

Additional Information

Alternative name: Grb2

Immunogen: Synthetic peptide corresponding to C-terminus of human Grb2 (amino acids 198-217).

Format: Purified

Description: Grb2 (growth factor receptor-bound protein 2) is a highly conserved adaptor proteincomposed of central SH2 domain flanked by two terminal SH3 domains. Grb2 mediatescoupling of receptor protein tyrosine kinases to a guanine nucleotide exchange factor Sos,thereby enabling the tyrosine kinases to stimulate activity of Ras and hence of MAP kinasepathway. Grb2 also can bind to receptor protein tyrosine phosphatases and affect theirsignaling.

Specificity: The polyclonal antibody recognizes Grb2, a highly conserved approximately 26 kDa adaptorprotein. The immunogen has 100% identity between man, cow, rat and mouse.

Synonyms: ASH, Grb3-3, MST084, NCKAP2, MSTP084, EGFRBP-GRB2, Ash, AA408164, MGC108668, zgc:73077, wu:fb18e12, wu:fb34e06, GRB2, MGC127576, DKFZp459P153, grb2, MGC64880, BB233594, 8430435N19Rik, MGC112733, GRAP, MGC166297, MGC110202, zgc:110202, MGC109892, si:dkeyp-9d4.1

Application Details

Application Notes: Western Blotting - Recommended dilution: 1 µg/ml. Positive control: RAMOS human Burkitt lymphoma cell line. Application note: Reducing conditions.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from rabbit serum by immunoaffinity chromatography.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Cancer

Restrictions: For Research Use only

Images

Western Blotting analysis (reducing conditions) of Ramos cell lysate using two batches of rabbit anti-Grb2 polyclonal (A, B).