HUWE1 antibody (C-Term)

Catalog No. ABIN302816

The Rabbit Polyclonal anti-HUWE1 antibody is suitable to detect HUWE1 in samples from Human, Mouse and Rat. It has been validated for WB and IHC (p).

Quick Overview for HUWE1 antibody (C-Term) (ABIN302816)

Target: HUWE1 (HECT, UBA and WWE Domain Containing 1, E3 Ubiquitin Protein Ligase (HUWE1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HUWE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HUWE1 Antibody

Binding Specificity: C-Term

Specificity: This antibody is specific to HUWE1/ARFBP1.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to a 19 amino acid peptide from near the carboxy terminus of ARF-BP1.

Isotype: IgG

Application Details

Application Notes: Western Blot: 2 μg/mL. Immunohistochemistry on Paraffin Sections: 20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for HUWE1

Target: HUWE1 (HECT, UBA and WWE Domain Containing 1, E3 Ubiquitin Protein Ligase (HUWE1))

Alternative Name: HUWE1 / UREB1

Background: ARFBP1 is a novel E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Also ubiquitinates the p53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene. Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN. May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation.Synonyms: ARF-BP1, ARF-binding protein 1, E3 ubiquitin-protein ligase HUWE1, HECT, HSPC272, HectH9, Homologous to E6AP carboxyl terminus homologous protein 9, KIAA0312, KIAA1578, LASU1, Large structure of UREB1, Mcl-1 ubiquitin ligase E3, Mule, UBA and WWE domain-containing protein 1, URE-B1, URE-binding protein 1, Upstream regulatory element-binding protein 1

Gene ID: 10075

NCBI Accession: NP_113584

UniProt: Q7Z6Z7