Merlin antibody (pSer518)

Catalog No. ABIN303117

The Rabbit Polyclonal anti-Merlin antibody (ABIN303117) specifically detects Merlin in WB, IP, IHC (p) and EIA. The antibody is reactive with Human and Mouse samples.

Quick Overview for Merlin antibody (pSer518) (ABIN303117)

Target: Merlin (NF2) (Neurofibromin 2 (NF2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Merlin antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Merlin Antibody

Binding Specificity: pSer518

Specificity: This antibody is specific for the phosphorylated form of Human NF2 (Merlin).

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human

Purification: Immunoaffinity Chromatography.

Immunogen: Human NF2 (Merlin) phospho-peptide corresponding to a region of the human protein surrounding S518 and conjugated to Keyhole Limpet Hemocyanin (KLH).

Application Details

Application Notes: ELISA. Western Blot. Immunoprecipitation. Immunohistochemistry on Paraffin Sections (2.5 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preserevative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer. Dilute only prior to immediate use.

Target Details for Merlin

Target: Merlin (NF2) (Neurofibromin 2 (NF2))

Alternative Name: Merlin

Background: The recently isolated neurofibromatosis 2 tumor suppressor gene encodes a 595 amino acid protein (Merlin). The protein product Merlin, named for its relatedness to the ezrin, radixin and moesin (ERM) family of proteins, is a tumor suppressor whose absence results in the occurrence of multiple tumors of the nervous system, particularly schwannomas and meningiomas. Merlin's similarity to ERMs suggests that it might share functions, acting as a link between cytoskeletal components and the cell membrane. The NF2 protein is highly expressed in human fibroblasts and is detected as a single band of about 75 kDa.Synonyms: Moesin-ezrin-radixin-like protein, NF2, Neurofibromin-2, SCH, Schwannomerlin, Schwannomerlin, Schwannomin, Schwannomin

Gene ID: 4771

NCBI Accession: NP_000259

UniProt: P35240

Pathways: Cell-Cell Junction Organization