IL-2 antibody (AA 21-155)

Catalog No. ABIN3042519

This anti-IL-2 antibody is a Rabbit Polyclonal antibody detecting IL-2 in WB. Suitable for Rat. This Primary Antibody has been cited in 7+ publications.

Quick Overview for IL-2 antibody (AA 21-155) (ABIN3042519)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-IL-2 Antibody

Binding Specificity: AA 21-155

Purpose: Anti-IL2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-IL2 Antibody Picoband® (ABIN3042519). Tested in WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived rat IL-2 recombinant protein (Position: A21-Q155). Rat IL-2 shares 66% and 80% amino acid (aa) sequences identity with human and mouse IL-2, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Rat
1. Hollander, G. A., Zuklys, S., Morel, C., Mizoguchi, E., Mobisson, K., Simpson, S., Terhorst, C., Wishart, W., Golan, D. E., Bhan, A. K., Burakoff, S. J. Monoallelic expression of the interleukin-2 locus. Science 279: 2118-2121, 1998. 2. Lowenthal, J. W., Zubler, R. H., Nabholz, M., MacDonald, H. R. Similarities between interleukin-2 receptor number and affinity on activated B and T lymphocytes. Nature 315: 669-672, 1985. 3. Shows, T., Eddy, R., Haley, L., Byers, M., Henry, M., Fujita, T., Matsui, H., Taniguchi, T. Interleukin 2 (IL2) is assigned to human chromosome 4. Somat. Cell Molec. Genet. 10: 315-318, 1984.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-IL-2 antibody (ABIN3042519)

Li, Wu, Hang, Zhang, Li: "Women with recurrent spontaneous abortion have decreased 25(OH) vitamin D and VDR at the fetal-maternal interface." in: Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, Vol. 50, Issue 11, pp. e6527, (2017) (PubMed).

Zheng, Wu, Yang, Huang, Chen, Gong, Fu, Lin, Tan: "Treatment of radiation-induced acute intestinal injury with bone marrow-derived mesenchymal stem cells." in: Experimental and therapeutic medicine, Vol. 11, Issue 6, pp. 2425-2431, (2016) (PubMed).

Hu, Jing, Wang, Zhang, Yong, Wang: "The positive effects of Ginsenoside Rg1 upon the hematopoietic microenvironment in a D-Galactose-induced aged rat model." in: BMC complementary and alternative medicine, Vol. 15, pp. 119, (2015) (PubMed).

Chen, Liu, Xu, Yang, Zhang, Zhang: "Effectiveness of immune therapy combined with chemotherapy on the immune function and recurrence rate of cervical cancer." in: Experimental and therapeutic medicine, Vol. 9, Issue 3, pp. 1063-1067, (2015) (PubMed).

Xie, Cao, Zhao, Yu, Qi, Dai: "IDO expressing dendritic cells suppress allograft rejection of small bowel transplantation in mice by expansion of Foxp3+ regulatory T cells." in: Transplant immunology, Vol. 33, Issue 2, pp. 69-77, (2015) (PubMed).

Gong, Wang, Yuan, Li, Gu, Zhao, Zhang, Jia, Feng, Liu: "Inhibition of Tumor Growth and Immunomodulatory Effects of Flavonoids and Scutebarbatines of Scutellaria barbata D. Don in Lewis-Bearing C57BL/6 Mice." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 630760, (2015) (PubMed).

Zhong, Shen, Sun, Wang, Zhang, Sun: "Additive effects of ulinastatin and docetaxel on growth of breast cancer xenograft in nude mice and expression of PGE2, IL-10, and IL-2 in primary breast cancer cells." in: Cancer biotherapy & radiopharmaceuticals, Vol. 27, Issue 4, pp. 252-8, (2012) (PubMed).

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL2

Background:

Synonyms: Interleukin-2,IL-2,T-cell growth factor,TCGF,Il2,Il-2,

Tissue Specificity: Nervous system.

Background: Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. This gene for interleukin 2 (IL2) is assigned to chromosome 4. IL-2 is a 15.5KDa glycoprotein, consisting of 153 amino acids in precursor form and 133 amino acids in mature form.

Sequence Similarities: Belongs to the CNTF family.

Molecular Weight: 18 kDa

Gene ID: 116562

UniProt: P17108

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation