Cx40/GJA5 antibody (C-Term)

Catalog No. ABIN3042610

Product Details anti-Cx40/GJA5 Antibody

Target: Cx40/GJA5 (GJA5) (Gap Junction Protein, alpha 5, 40kDa (GJA5))

Binding Specificity: AA 341-358, C-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cx40/GJA5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Gap junction alpha-5 protein(GJA5) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: KRRLSKASSK ARSDDLSV

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Gap junction alpha-5 protein(GJA5) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: gap junction protein, alpha 5, 40 kDa
Protein Name: Gap junction alpha-5 protein

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Connexin 40/GJA5(341-358aa KRRLSKASSKARSDDLSV), identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Predicted Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Cx40/GJA5

Target: Cx40/GJA5 (GJA5) (Gap Junction Protein, alpha 5, 40kDa (GJA5))

Alternative Name: GJA5 (GJA5 Products)

Synonyms: Cx40 antibody, 5730555N10Rik antibody, Cnx40 antibody, Gja-5 antibody, ATFB11 antibody, CX40 antibody, gap junction protein, alpha 5 antibody, gap junction protein alpha 5 antibody, Gja5 antibody, GJA5 antibody

Background: Connexin proteins oligomerize to form intercellular channels, called gap junctions, through which ions and small molecules move between adjacent cells. Connexin 40(Gap junction alpha-5 protein) is a protein that in humans is encoded by the GJA5 gene.This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene may be associated with atrial fibrillation. Alternatively spliced transcript variants encoding the same isoform have been described.

Synonyms: Connexin 40 antibody|Connexin-40 antibody|Cx40 antibody|CXA5_HUMAN antibody|Gap junction alpha 5 protein antibody|Gap junction alpha-5 protein antibody|Gap junction protein alpha 5 40kD(connexin 40) antibody|Gap junction protein alpha 5 antibody|GJA5 antibody|MGC11185 antibody

UniProt: P36382

Pathways: Cell-Cell Junction Organization