EPH Receptor B3 antibody (C-Term)

Catalog No. ABIN3042663

Product Details anti-EPH Receptor B3 Antibody

Target: EPH Receptor B3 (EPHB3)

Binding Specificity: AA 982-998, C-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPH Receptor B3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Ephrin type-B receptor 3(EPHB3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: SIQDMRLQMN QTLPVQV

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Ephrin type-B receptor 3(EPHB3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: EPH receptor B3
Protein Name: Ephrin type-B receptor 3

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Eph receptor B3(982-998aa SIQDMRLQMNQTLPVQV), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for EPH Receptor B3

Target: EPH Receptor B3 (EPHB3)

Alternative Name: EPHB3 (EPHB3 Products)

Synonyms: tck antibody, MGC83457 antibody, EPHB3 antibody, ETK2 antibody, HEK2 antibody, TYRO6 antibody, AW456895 antibody, Cek10 antibody, Etk2 antibody, MDK5 antibody, Sek4 antibody, Tyro6 antibody, CEK10 antibody, EK10 antibody, ek3 antibody, ephb3 antibody, fc62d03 antibody, rtk3 antibody, wu:fc62d03 antibody, zek3 antibody, EPH receptor B3 antibody, EPH receptor B3 L homeolog antibody, Eph receptor B3 antibody, eph receptor B3a antibody, Eph receptor tyrosine kinase antibody, EPHB3 antibody, ephb3.L antibody, ephb3 antibody, Ephb3 antibody, ephb3a antibody

Background: Ephrin Receptor EphB3, is also known as human embryo kinase2(HEK2) or Eph-like tyrosine kinase2(ETK2). HEK2, which is a member of the EPH/ELK family of tyrosine kinases, encodes a 998-amino acid polypeptide having a single putative transmembrane domain, a secretory signal sequence, and 2 fibronectin repeats. The EPHB3 gene is mapped to human chromosome 3q21-qter. HEK2 interacts with 2 ligands of EPH-related kinases(LERKs), namely, LERK2(EFNB1) and LERK5(EFNB2). Coincubation of HEK2- and LERK2-expressing cells induces cell-cell adhesion and aggregation. Additionally, coexpression of HEK2 and LERK2 results in reduced kinase activity of HEK2.

Synonyms: Cek10 antibody|EK2 antibody|Embryonic kinase 2 antibody|EPH Like Tyrosine Kinase 2 antibody|EPH-like kinase 2 antibody|EphB3 antibody|EPHB3_HUMAN antibody|Ephrin receptor EphB3 antibody|Ephrin type B receptor 3 antibody|Ephrin type-B receptor 3 antibody|ETK2 antibody|HEK2 antibody|Human Embryo Kinase 2 antibody|Mdk5 antibody|Sek4 antibody|TYRO6 antibody|Tyrosine protein kinase receptor HEK2 antibody|Tyrosine protein kinase TYRO6 antibody|Tyrosine-protein kinase TYRO6 antibody

UniProt: P54753

Pathways: RTK Signaling