EGF antibody (Epidermal Growth Factor) (AA 1013-1029)

Details for Product anti-EGF Antibody No. ABIN3042717, Supplier: Log in to see
Antigen
  • HOMG4
  • URG
  • AI790464
  • CEGF
  • epidermal growth factor
  • pro-epidermal growth factor
  • EGF
  • egf
  • CpipJ_CPIJ020278
  • Egf
  • LOC100008808
Alternatives
anti-Rat (Rattus) EGF antibody for Immunohistochemistry (Frozen Sections)
Epitope
AA 1013-1029, C-Term
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Reactivity
Mouse (Murine)
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Host
Rabbit
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Clonality
Polyclonal
Conjugate
This EGF antibody is un-conjugated
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Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Options
Supplier
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Supplier Product No.
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Purpose Rabbit IgG polyclonal antibody for Pro-epidermal growth factor(EGF) detection. Tested with WB, IHC-P in Mouse.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of mouse EGF(1013-1029aa YSGDRCQTRDLRWWELR).
Sequence YSGDRCQTRD LRWWELR
Isotype IgG
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Pro-epidermal growth factor(EGF) detection. Tested with WB, IHC-P in Mouse.
Gene Name: epidermal growth factor
Protein Name: Pro-epidermal growth factor(EGF)
Purification Immunogen affinity purified.
Alternative Name EGF (EGF Antibody Abstract)
Background EGF is known as epidermal growth factor. This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. Additionally, it acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

Synonyms: Beta urogastrone antibody|EGF antibody|Egf antibody|EGF_HUMAN antibody|Epidermal Growth Factor antibody|Epidermal Growth Factor antibody|HOMG4 antibody|OTTHUMP00000219721 antibody|OTTHUMP00000219722 antibody|Pro epidermal growth factor antibody|URG antibody|Urogastrone antibody
UniProt P01132
Research Area Cancer, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Epidermal Growth Factor (EGF) (AA 1013-1029), (C-Term) antibody (ABIN3042717) Anti-EGF antibody, Western blotting Lane 1: Recombinant Mouse EGF Protein 10ng Lane 2...
Immunohistochemistry (IHC) image for anti-Epidermal Growth Factor (EGF) (AA 1013-1029), (C-Term) antibody (ABIN3042717) Anti-EGF antibody, IHC(P) IHC(P): Mouse Intestine Tissue
Product cited in: Ruan, Jin, Zhang, Wang, Chen, Ding, Wen: "Peptide-chaperone-directed transdermal protein delivery requires energy." in: Molecular pharmaceutics, Vol. 11, Issue 11, pp. 4015-22, 2014 (PubMed).

Ge, Yu, Petitte, Zhang: "Epidermal growth factor-induced proliferation of chicken primordial germ cells: involvement of calcium/protein kinase C and NFKB1." in: Biology of reproduction, Vol. 80, Issue 3, pp. 528-36, 2009 (PubMed).

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