Cullin 3 antibody (Middle Region)

Catalog No. ABIN3042989

Product Details anti-Cullin 3 Antibody

Target: Cullin 3 (CUL3)

Binding Specificity: AA 426-444, Middle Region

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cullin 3 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Cullin-3(CUL3) detection. Tested with WB in Human,Mouse,Rat.

Sequence: DVFERYYKQH LARRLLTNK

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Cullin-3(CUL3) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: cullin 3
Protein Name: Cullin-3

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Cullin 3(426-444aa DVFERYYKQHLARRLLTNK), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for CUL3 is approximately 0.5 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Cullin 3

Target: Cullin 3 (CUL3)

Alternative Name: CUL3 (CUL3 Products)

Synonyms: CUL-3 antibody, Cullin-3 antibody, CUL-3-A antibody, Cullin-3-A antibody, cul3 antibody, cul3a antibody, CUL-3-B antibody, Cullin-3-B antibody, cul3b antibody, wu:fc37e01 antibody, CUL3 antibody, ATCUL3 antibody, ATCUL3A antibody, CUL3A antibody, T24P13.25 antibody, cullin 3 antibody, cullin 3A antibody, PHA2E antibody, AI467304 antibody, AW146203 antibody, mKIAA0617 antibody, cullin 3 antibody, cullin 3 L homeolog antibody, cullin 3 S homeolog antibody, cullin-3 antibody, Cullin-3 (CUL-3) antibody, cullin 3a antibody, CULlin antibody, CUL3 antibody, cul3 antibody, cul3.L antibody, cul3.S antibody, PTRG_11586 antibody, Smp_117580 antibody, cul3a antibody, Cul3 antibody, cul-3 antibody

Background: Cullin-3(Cul 3), is a protein that in humans is encoded by the CUL3 gene. Kipreos et al.(1996) identified a conserved gene family, designated cullins, with at least 5 members in nematodes, 6 in humans, and 3 in S. cerevisiae. Human CUL3 is an ortholog of nematode cul3. Hartz(2012) mapped the CUL3 gene to chromosome 2q36.2 based on an alignment of the CUL3 sequence with the genomic sequence(GRCh37). Sumara et al.(2007) found that KLHL9, KLHL13, and CUL3 interacted directly in a 370-kD protein complex in HeLa cell lysates. Rondou et al.(2008) showed that interaction between KLHL12 and the CUL3 ubiquitin ligase complex directed ubiquitination of dopamine receptor D4(DRD4). KLHL12 interacted directly with CUL3 and with the polymorphic intracellular loop-3 of D4. By immunoprecipitation analysis of HeLa cells and HCT116 human colon carcinoma cells expressing epitope-tagged proteins, Kigoshi et al.(2011) showed that KLHL7 interacted with CUL3 and ROC1(RBX1).

Synonyms: CUL 3 antibody|Cul-3 antibody|CUL3 antibody|CUL3_HUMAN antibody|Cullin-3 antibody|Cullin3 antibody|KIAA0617 antibody

UniProt: Q13618

Pathways: M Phase