ENO2/NSE antibody (C-Term)
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- Target See all ENO2/NSE (ENO2) Antibodies
- ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
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Binding Specificity
- AA 420-434, C-Term
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This ENO2/NSE antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- Rabbit IgG polyclonal antibody for Gamma-enolase(ENO2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- Sequence
- EARFAGHNFR NPSVL
- Cross-Reactivity (Details)
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Predicted Cross Reactivity: human, mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities. - Characteristics
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Rabbit IgG polyclonal antibody for Gamma-enolase(ENO2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: enolase 2(gamma, neuronal)
Protein Name: Gamma-enolase - Purification
- Immunogen affinity purified.
- Immunogen
- A synthetic peptide corresponding to a sequence at the C-terminus of human NSE(420-434aa EARFAGHNFRNPSVL), identical to the related mouse and rat sequences.
- Isotype
- IgG
- Top Product
- Discover our top product ENO2 Primary Antibody
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- Application Notes
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- Preservative
- Thimerosal (Merthiolate), Sodium azide
- Precaution of Use
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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: "In Vitro Differentiation of Bone Marrow Mesenchymal Stem Cells into Neuron-Like Cells by Cerebrospinal Fluid Improves Motor Function of Middle Cerebral Artery Occlusion Rats." in: Frontiers in neurology, Vol. 7, pp. 183, (2016) (PubMed).
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: "Bone marrow-derived mesenchymal stem cells differentiate into nerve-like cells in vitro after transfection with brain-derived neurotrophic factor gene." in: In vitro cellular & developmental biology. Animal, Vol. 51, Issue 3, pp. 319-27, (2015) (PubMed).
: "Methyl 3,4-dihydroxybenzoate promotes neurite outgrowth of cortical neurons cultured in vitro." in: Neural regeneration research, Vol. 7, Issue 13, pp. 971-7, (2015) (PubMed).
: "Synergistic effects of Buyang Huanwu decoction and embryonic neural stem cell transplantation on the recovery of neurological function in a rat model of spinal cord injury." in: Experimental and therapeutic medicine, Vol. 9, Issue 4, pp. 1141-1148, (2015) (PubMed).
: "Anti-epileptic effect of Ganoderma lucidum polysaccharides by inhibition of intracellular calcium accumulation and stimulation of expression of CaMKII α in epileptic hippocampal neurons." in: PLoS ONE, Vol. 9, Issue 7, pp. e102161, (2015) (PubMed).
: "Effects of transforming growth factor-?2 on myocilin expression and secretion in human primary cultured trabecular meshwork cells." in: International journal of clinical and experimental pathology, Vol. 7, Issue 8, pp. 4827-36, (2014) (PubMed).
: "Effects of rhynchophylline on GluN1 and GluN2B expressions in primary cultured hippocampal neurons." in: Fitoterapia, Vol. 98, pp. 166-73, (2014) (PubMed).
: "Using microfluidic chip to form brain-derived neurotrophic factor concentration gradient for studying neuron axon guidance." in: Biomicrofluidics, Vol. 8, Issue 1, pp. 14108, (2014) (PubMed).
: "Amyloid beta-derived diffusible ligands (ADDLs) induce abnormal expression of insulin receptors in rat hippocampal neurons." in: Journal of molecular neuroscience : MN, Vol. 52, Issue 1, pp. 124-30, (2014) (PubMed).
: "BDNF attenuates IL-1?-induced F-actin remodeling by inhibiting NF-?B signaling in hippocampal neurons." in: Neuro endocrinology letters, Vol. 35, Issue 1, pp. 13-9, (2014) (PubMed).
: "Neuroprotective effect of Atractylodes macrocephalaon polysaccharides in vitro on neuronal apoptosis induced by hypoxia." in: Molecular medicine reports, Vol. 9, Issue 6, pp. 2573-81, (2014) (PubMed).
: "Anticancer activity of SAHA, a potent histone deacetylase inhibitor, in NCI-H460 human large-cell lung carcinoma cells in vitro and in vivo." in: International journal of oncology, Vol. 44, Issue 2, pp. 451-8, (2014) (PubMed).
: "Investigation of characteristics of feather follicle stem cells and their regeneration potential." in: Journal of stem cells & regenerative medicine, Vol. 7, Issue 2, pp. 69-74, (2014) (PubMed).
: "Injury of cortical neurons is caused by the advanced glycation end products-mediated pathway." in: Neural regeneration research, Vol. 8, Issue 10, pp. 909-15, (2014) (PubMed).
: "Optimal therapeutic dose and time window of picroside II in cerebral ischemic injury." in: Neural regeneration research, Vol. 9, Issue 15, pp. 1437-45, (2014) (PubMed).
: "Cholinergic neuron-like cells derived from bone marrow stromal cells induced by tricyclodecane-9-yl-xanthogenate promote functional recovery and neural protection after spinal cord injury." in: Cell transplantation, Vol. 22, Issue 6, pp. 961-75, (2013) (PubMed).
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The association and significance of H3K27me3 and a folate metabolic gene ACat2 in neural tube defects." in: Nutrition journal, Vol. 15, Issue 1, pp. 95, (2017) (PubMed).
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- Target
- ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
- Alternative Name
- ENO2 (ENO2 Products)
- Synonyms
- ENO2 antibody, DKFZp459B1817 antibody, NSE antibody, AI837106 antibody, D6Ertd375e antibody, Eno-2 antibody, RNEN3 antibody, eno3 antibody, wu:fc09h05 antibody, zgc:92418 antibody, enolase 2 antibody, enolase 2 (gamma, neuronal) antibody, enolase 2, gamma neuronal antibody, enolase 2, gamma, neuronal antibody, ENO2 antibody, Eno2 antibody, eno2 antibody
- Background
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NSE(neuron specific enolase), also known as Enolase 2(ENO2), is found in elevated concentrations in plasma in certain neoplasias. The enolases catalyze the interconversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. ENO2 gene contains 12 exons distributed over 9,213 nucleotides. Human neurone-specific enolase is mapped to chromosome 12p13.
Synonyms: 2 phospho D glycerate hydrolyase antibody|2-phospho-D-glycerate hydro-lyase antibody|Eno 2 antibody|Eno2 antibody|ENOG antibody|ENOG_HUMAN antibody|Enolase 2 antibody|Enolase 2 gamma neuronal antibody|Enolase2 antibody|Gamma enolase antibody|Gamma-enolase antibody|Neural enolase antibody|Neuron specific enolase antibody|Neuron specific gamma enolase antibody|Neuron-specific enolase antibody|Neurone specific enolase antibody|NSE antibody - UniProt
- P09104
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