LIF antibody (AA 23-202)

Catalog No. ABIN3043403

Product Details anti-LIF Antibody

Target: LIF (Leukemia Inhibitory Factor (LIF))

Binding Specificity: AA 23-202

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Leukemia inhibitory factor(LIF) detection. Tested with WB, IHC-P in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Leukemia inhibitory factor(LIF) detection. Tested with WB, IHC-P in Human.
Gene Name: leukemia inhibitory factor
Protein Name: Leukemia inhibitory factor

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human LIF recombinant protein (Position: S23-F202). Human LIF shares 78% and 82% amino acid (aa) sequences identity with mouse and rat LIF, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for LIF is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-LIF antibody (ABIN3043403)

Zhang, Liu, Liu, Zhou, Song, Cao, An: "miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression." in: PLoS ONE, Vol. 12, Issue 7, pp. e0179783, (2017) (PubMed).

Dong, Sui, Huang, Wang, Hu, Xiong, Wang, Zhang: "MicroRNA-223-3p suppresses leukemia inhibitory factor expression and pinopodes formation during embryo implantation in mice." in: American journal of translational research, Vol. 8, Issue 2, pp. 1155-63, (2016) (PubMed).

Gui, Xiong, Yang, Li, Huang: "Effects of acupuncture on LIF and IL-12 in rats of implantation failure." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 67, Issue 5, pp. 383-90, (2012) (PubMed).

Xu, Sun, Li, Wu, Zhang, Feng: "Pinopodes, leukemia inhibitory factor, integrin-?3, and mucin-1 expression in the peri-implantation endometrium of women with unexplained recurrent pregnancy loss." in: Fertility and sterility, Vol. 98, Issue 2, pp. 389-95, (2012) (PubMed).

Li, Xu, Chen, Sun: "Effects of hydrosalpinx on pinopodes, leukaemia inhibitory factor, integrin beta3 and MUC1 expression in the peri-implantation endometrium." in: European journal of obstetrics, gynecology, and reproductive biology, Vol. 151, Issue 2, pp. 171-5, (2010) (PubMed).

Pei, Yu, Shi, Jia: "The effects of mifepristone on the expressions of osteopontin, interleukin-6 and leukemia inhibitory factor in the villi of early pregnant women." in: Contraception, Vol. 82, Issue 4, pp. 379-84, (2010) (PubMed).

Hu, Feng, Tang, Zhu, Lin, Yu: "LIF upregulates expression of NK-1R in NHBE cells." in: Mediators of inflammation, Vol. 2006, Issue 5, pp. 84829, (2007) (PubMed).

Target Details for LIF

Target: LIF (Leukemia Inhibitory Factor (LIF))

Alternative Name: LIF (LIF Products)

Synonyms: CDF antibody, DIA antibody, HILDA antibody, MLPLI antibody, LIF, interleukin 6 family cytokine antibody, leukemia inhibitory factor antibody, LIF antibody, Lif antibody

Background: LIF is a pleiotropic cytokine produced at the maternal-fetal interface which has been shown to play an essential role in implantation in mice. This gene is mapped to 22q11-q12.2, between the Philadelphia translocation BCR gene and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF is produced in high amounts by the human endometrium and the trophoblast itself, and LIF receptors are present on cytotrophoblast cells. LIF could, thus, play a role in modulating HLA-G production and immune tolerance at the maternal-fetal interface.

Synonyms: CDF antibody|Cholinergic Differentiation Factor antibody|D factor antibody|DIA antibody|Differentiation inducing factor antibody|differentiation inhibitory activity antibody|Differentiation stimulating factor antibody|Differentiation-stimulating factor antibody|Emfilermin antibody|Hepatocyte stimulating factor III antibody|HILDA antibody|Human interleukin in DA cells antibody|Leukemia inhibitory factor antibody|LIF antibody|LIF_HUMAN antibody|Melanoma derived LPL inhibitor antibody|Melanoma-derived LPL inhibitor antibody|MLPLI antibody

Gene ID: 3976

UniProt: P15018

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding