RAB3A antibody (C-Term)

Catalog No. ABIN3043559

Product Details anti-RAB3A Antibody

Target: RAB3A (RAB3A, Member RAS Oncogene Family (RAB3A))

Binding Specificity: AA 192-220, C-Term

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAB3A antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Ras-related protein Rab-3A(RAB3A) detection. Tested with WB in Human,Mouse,Rat.

Sequence: DTADPAVTGA KQGPQLSDQQ VPPHQDCAC

Cross-Reactivity (Details): Predicted Cross Reactivity: human
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Ras-related protein Rab-3A(RAB3A) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: RAB3A, member RAS oncogene family
Protein Name: Ras-related protein Rab-3A

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Rab3A (192-220aa DTADPAVTGAKQGPQLSDQQVPPHQDCAC), different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human, The detection limit for Rab3A is approximately 0.1 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for RAB3A

Target: RAB3A (RAB3A, Member RAS Oncogene Family (RAB3A))

Alternative Name: RAB3A (RAB3A Products)

Synonyms: RAB3 antibody, rab3a antibody, zgc:92276 antibody, RAB3A antibody, rab-3a antibody, si:ch211-255d18.4 antibody, zgc:112516 antibody, RAB3A, member RAS oncogene family antibody, RAB3A, member RAS oncogene family, a antibody, RAB3A, member RAS oncogene family L homeolog antibody, RAB3A, member RAS oncogene family, b antibody, RAB3A antibody, Rab3a antibody, rab3aa antibody, rab3a.L antibody, rab3a antibody, rab3ab antibody

Background: Ras-related protein Rab-3A is a protein that in humans is encoded by the RAB3A gene. This gene is mapped to 19p13.11. Synapsin I stimulated the RAB3A cycle by increasing GTP binding, GTPase activity, and RAB3A recruitment to the synaptic vesicle membrane. Conversely, RAB3A inhibited synapsin I binding to actin and synapsin I-induced synaptic vesicle clustering. It has been found that RAB3A was necessary for both forms of synaptic plasticity, and it is also involved in calcium exocytosis in neurons. In addition to that, RAB3A has been found to regulate a late step in synaptic vesicle fusion.

Synonyms: Rab 3A antibody|RAB 3A member RAS oncogene family antibody|Rab3a antibody|RAB3A member RAS oncogene family antibody|RAB3A_HUMAN antibody|RAS associated protein RAB 3A antibody|RAS associated protein RAB3A antibody|Ras related protein Rab 3A antibody|Ras related protein Rab3A antibody|Ras-related protein Rab-3A antibody

Gene ID: 5864

UniProt: P20336

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport