HRH3 antibody (C-Term)

Catalog No. ABIN3043704

Product Details anti-HRH3 Antibody

Target: HRH3 (Histamine Receptor H3 (HRH3))

Binding Specificity: AA 428-445, C-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRH3 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Histamine H3 receptor(HRH3) detection. Tested with WB in Human,Mouse,Rat.

Sequence: CPQKLKIQPH SSLEHCWK

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Histamine H3 receptor(HRH3) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: histamine receptor H3
Protein Name: Histamine H3 receptor(H3R/HH3R)

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HRH3(428-445aa CPQKLKIQPHSSLEHCWK), different from the related rat sequence by three amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for HRH3

Target: HRH3 (Histamine Receptor H3 (HRH3))

Alternative Name: HRH3 (HRH3 Products)

Synonyms: GPCR97 antibody, HH3R antibody, AW049250 antibody, H3R antibody, histamine receptor H3 antibody, HRH3 antibody, Hrh3 antibody

Background: The histamine receptor H3(HRH3) is a presynaptic autoreceptor on histamine neurons in the brain and a presynaptic heteroreceptor in nonhistamine-containing neurons in both the central and peripheral nervous systems. The deduced 445-amino acid HRH3 protein contains 7 predicted transmembrane domains. And it shares 22 % and 21.4 % amino acid sequence homology with the H1(HRH1) and H2(HRH2) receptors, respectively. The expression of recombinant HRH3 in a variety of cell lines conferred an ability to inhibit adenylate cyclase in response to histamine, but not to acetylcholine or any other biogenic amine. Additionally, HRH3 was most notably observed throughout the thalamus, the ventromedial hypothalamus, and the caudate nucleus. Strong expression was also seen in layers II, V, and VIb of the cerebral cortex, in the pyramidal layers of the hippocampus, and in olfactory tubercle.

Synonyms: G protein coupled receptor 97 antibody|G-protein coupled receptor 97 antibody|GPCR 97 antibody|GPCR97 antibody|H3R antibody|HH 3R antibody|HH3 R antibody|HH3R antibody|Histamine H3 receptor antibody|Histamine receptor H3 antibody|HRH 3 antibody|HRH3 antibody|HRH3_HUMAN antibody|OTTHUMP00000031459 antibody

UniProt: Q9Y5N1

Pathways: cAMP Metabolic Process, Feeding Behaviour