Aryl Hydrocarbon Receptor antibody (Middle Region)

Catalog No. ABIN3043778

Product Details anti-Aryl Hydrocarbon Receptor Antibody

Target: Aryl Hydrocarbon Receptor (AHR)

Binding Specificity: AA 525-552, Middle Region

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aryl Hydrocarbon Receptor antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Aryl hydrocarbon receptor(AHR) detection. Tested with WB in Mouse,Rat.

Sequence: DNKNNDLYNI MRNLGIDFED IRSMQNEE

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Aryl hydrocarbon receptor(AHR) detection. Tested with WB in Mouse,Rat.
Gene Name: aryl hydrocarbon receptor
Protein Name: Aryl hydrocarbon receptor

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of mouse AHR (525-552aa DNKNNDLYNIMRNLGIDFEDIRSMQNEE), different from the related human sequence by five amino acids, and from the related rat sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Rat, The detection limit for AHR is approximately 0.1 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for Aryl Hydrocarbon Receptor

Target: Aryl Hydrocarbon Receptor (AHR)

Alternative Name: AHR (AHR Products)

Synonyms: AHR antibody, ahr1 antibody, ahr antibody, bHLHe76 antibody, Ah antibody, Ahh antibody, Ahre antibody, In antibody, aryl hydrocarbon receptor S homeolog antibody, aryl hydrocarbon receptor antibody, aryl-hydrocarbon receptor antibody, ahr.S antibody, AHR antibody, ahr antibody, Ahr antibody

Background: AHR (aryl hydrocarbon receptor), also called bHLHe76, is a member of the family of basic helix-loop-helix transcription factors. AhR is a cytosolic transcription factor that is normally inactive, bound to several co-chaperones. The AHR gene is mapped on 7p21.1. Estrogenic actions of AHR agonists were detected in wildtype ovariectomized mouse uteri, but were absent in Ahr -/- or Er-alpha -/- ovariectomized mice. Complex assembly and ubiquitin ligase activity of CUL4B (AHR) in vitro and in vivo are dependent on the AHR ligand. In the CUL4B (AHR) complex, ligand-activated AHR acts as a substrate-specific adaptor component that targets sex steroid receptors for degradation. Cd4-positive cells from mice lacking Ahr developed Th17 responses but failed to produce Il22 and did not show enhanced Th17 development. Activation of Ahr during induction of EAE accelerated disease onset and increased pathology in wildtype mice, but not in Ahr -/- mice. The TDO-AHR pathway is active in human brain tumors and is associated with malignant progression and poor survival. Ahr activity within ROR-gamma-t-positive ILC could be induced by dietary ligands such as those contained in vegetables of the family Brassicaceae.

Synonyms: Ah receptor antibody|AHR antibody|AHR_HUMAN antibody|Aromatic hydrocarbon receptor antibody|Aryl hydrocarbon receptor antibody|Aryl hydrocarbon receptor precursor antibody| bHLHe76 antibody|Class E basic helix loop helix protein 76 antibody|Class E basic helix-loop-helix protein 76 antibody|HGNC:348 antibody

Gene ID: 11622

UniProt: P30561

Pathways: Regulation of Cell Size