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GCN2 antibody (Middle Region)

EIF2AK4 Reactivity: Mouse WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN3044057
  • Target See all GCN2 (EIF2AK4) Antibodies
    GCN2 (EIF2AK4) (Eukaryotic Translation Initiation Factor 2 alpha Kinase 4 (EIF2AK4))
    Binding Specificity
    • 19
    • 15
    • 10
    • 9
    • 8
    • 6
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 868-886, Middle Region
    Reactivity
    • 57
    • 42
    • 22
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    • 82
    • 3
    • 2
    Rabbit
    Clonality
    • 77
    • 10
    Polyclonal
    Conjugate
    • 32
    • 8
    • 7
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GCN2 antibody is un-conjugated
    Application
    • 73
    • 43
    • 38
    • 15
    • 14
    • 14
    • 10
    • 8
    • 8
    • 6
    • 3
    • 1
    • 1
    Western Blotting (WB)
    Purpose
    Rabbit IgG polyclonal antibody for eIF-2-alpha kinase GCN2(EIF2AK4) detection. Tested with WB in Mouse,Rat.
    Sequence
    ATDHLAFTAE GKQDDQAGD
    Cross-Reactivity (Details)
    Predicted Cross Reactivity: rat
    No cross reactivity with other proteins.
    Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
    Characteristics
    Rabbit IgG polyclonal antibody for eIF-2-alpha kinase GCN2(EIF2AK4) detection. Tested with WB in Mouse,Rat.
    Gene Name: eukaryotic translation initiation factor 2 alpha kinase 4
    Protein Name: eIF-2-alpha kinase GCN2
    Purification
    Immunogen affinity purified.
    Immunogen
    A synthetic peptide corresponding to a sequence in the middle region of mouse GCN2(868-886aa ATDHLAFTAEGKQDDQAGD), different from the related rat sequence by one amino acid.
    Isotype
    IgG
    Top Product
    Discover our top product EIF2AK4 Primary Antibody
  • Application Notes
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Predicted Species: Rat, The detection limit for EIF2AK4 is approximately 0.5 ng/lane under reducing conditions.
    Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
    Other applications have not been tested. Optimal dilutions should be determined by end users.
    Comment

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Concentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
    Preservative
    Thimerosal (Merthiolate), Sodium azide
    Precaution of Use
    This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
    Expiry Date
    12 months
  • Target
    GCN2 (EIF2AK4) (Eukaryotic Translation Initiation Factor 2 alpha Kinase 4 (EIF2AK4))
    Alternative Name
    EIF2AK4 (EIF2AK4 Products)
    Synonyms
    EIF2AK4 antibody, Gcn2-like antibody, GCN2 antibody, 2610011M03 antibody, MGCN2 antibody, eukaryotic translation initiation factor 2 alpha kinase 4 antibody, eukaryotic translation initiation factor 2-alpha kinase 4 antibody, eIF-2-alpha kinase GCN2 antibody, EIF2AK4 antibody, LOC579979 antibody, LOC100187226 antibody, eif2ak4 antibody, Eif2ak4 antibody
    Background
    EIF2AK4(Eukaryotic Translation Initiation Factor 2-Alpha Kinase 4), also called GCN2, is an enzyme that in humans is encoded by the EIF2AK4 gene. EIF2AK4 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 to downregulate protein synthesis in response to varied cellular stresses. Hartz(2005) mapped the EIF2AK4 gene to chromosome 15q15.1 based on an alignment of the EIF2AK4 sequence with the genomic sequence. Berlanga et al.(1999) demonstrated that Gcn2 immunopurified from mouse liver extracts could phosphorylate rabbit Eif2 in vitro. Serum starvation increased the level of phosphorylated EIF2-alpha more than 2-fold in human embryonic kidney cells transfected with mouse Eif2ak4. Costa-Mattioli et al.(2005) reported a unique feature of hippocampal slices from Gcn2-null mice: in CA1, a single 100-Hz train induced a strong and sustained long-term potentiation(late LTP or L-LTP), which was dependent on transcription and translation.

    Synonyms: E2AK4_HUMAN antibody|Eif2ak4 antibody|Eukaryotic Translation Initiation Factor 2 alpha kinase 4 antibody|Eukaryotic translation initiation factor 2-alpha kinase 4 antibody|GCN2 antibody|GCN2 eIF2alpha kinase antibody|GCN2 like protein antibody|GCN2-like protein antibody|KIAA1338 antibody|MGCN2 antibody
    Pathways
    ER-Nucleus Signaling, Hepatitis C
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