HSD17B2 antibody (C-Term)

Catalog No. ABIN3044214

Product Details anti-HSD17B2 Antibody

Target: HSD17B2 (Hydroxysteroid (17-Beta) Dehydrogenase 2 (HSD17B2))

Binding Specificity: AA 373-387, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSD17B2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Rabbit IgG polyclonal antibody for Estradiol 17-beta-dehydrogenase 2(HSD17B2) detection. Tested with WB, IHC-P, IHC-F in Human.

Sequence: MPRALRMPNY KKKAT

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Estradiol 17-beta-dehydrogenase 2(HSD17B2) detection. Tested with WB, IHC-P, IHC-F in Human.
Gene Name: hydroxysteroid(17-beta) dehydrogenase 2
Protein Name: Estradiol 17-beta-dehydrogenase 2

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HSD17B2(373-387aa MPRALRMPNYKKKAT).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for HSD17B2

Target: HSD17B2 (Hydroxysteroid (17-Beta) Dehydrogenase 2 (HSD17B2))

Alternative Name: HSD17B2 (HSD17B2 Products)

Synonyms: EDH17B2 antibody, HSD17 antibody, SDR9C2 antibody, AI194836 antibody, AI194967 antibody, AI255511 antibody, hydroxysteroid 17-beta dehydrogenase 2 antibody, hydroxysteroid (17-beta) dehydrogenase 2 antibody, HSD17B2 antibody, Hsd17b2 antibody, hsd17b2 antibody

Background: HSD17B2(17-BETA-HYDROXYSTEROID DEHYDROGENASE II), also called 17-BETA-HSD II, is an anzyme which have 387 amino acids with a predicted molecular weight of 42,782 and associate with the membranes of the endoplasmic reticulum. Its Cytogenetic location is 16q23.3. The type 2 enzyme was capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. HSD17B2 mRNA was detected in 18 of 42(43 % ) adenomas but not in prolactinomas. In the human endometrium, inactivation of 17-beta-estradiol to estrone is catalyzed by HSD17B2. And HSD17B2 activity distinguishing between disease-free and diseased endometria. HSD17B2 efficiently catalyzes the oxidative metabolism of androgens and estrogens, and it is expressed in a large series of human peripheral tissues. The previous paradigm that HSD17B2 activity in the endometrium is elevated during the secretory phase is confined to diseased endometrium but not to disease-free endometrium.

Synonyms: 17 beta HSD 2 antibody|17-beta-HSD 2 antibody|17-beta-hydroxysteroid dehydrogenase type 2 antibody|20 alpha HSD antibody|20 alpha hydroxysteroid dehydrogenase antibody|20 alpha-hydroxysteroid dehydrogenase antibody|20-alpha-HSD antibody|DHB2_HUMAN antibody|E2DH antibody|EDH17B2 antibody|Estradiol 17-beta-dehydrogenase 2 antibody|HSD17 antibody|Hsd17b2 antibody|Hydroxysteroid(17 beta) dehydrogenase 2 antibody|Microsomal 17 beta hydroxysteroid dehydrogenase antibody|Microsomal 17-beta-hydroxysteroid dehydrogenase antibody|SDR9C2 antibody|Testosterone 17 beta dehydrogenase antibody|Testosterone 17-beta-dehydrogenase antibody

UniProt: P37059

Pathways: Steroid Hormone Biosynthesis