EPAS1 antibody (Middle Region)

Catalog No. ABIN5518650

Product Details anti-EPAS1 Antibody

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Binding Specificity: AA 282-301, Middle Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPAS1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Endothelial PAS domain-containing protein 1(EPAS1) detection. Tested with WB in Human,Mouse,Rat.

Sequence: HALDSENMTK SHQNLCTKGQ

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Endothelial PAS domain-containing protein 1(EPAS1) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: endothelial PAS domain protein 1
Protein Name: Endothelial PAS domain-containing protein 1(EPAS-1)

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human HIF-2-alpha(282-301aa, HALDSENMTKSHQNLCTKGQ), identical to the related mouse and rat sequence.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-EPAS1 antibody (ABIN5518650)

Lu, Shi, Ma, Wang, Liu, Chuai, Song, Münsterberg, Cao, Yang: "Proper autophagy is indispensable for angiogenesis during chick embryo development." in: Cell cycle (Georgetown, Tex.), Vol. 15, Issue 13, pp. 1742-54, (2017) (PubMed).

Target Details for EPAS1

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Alternative Name: EPAS1 (EPAS1 Products)

Synonyms: EPAS1 antibody, ECYT4 antibody, HIF2A antibody, HLF antibody, MOP2 antibody, PASD2 antibody, bHLHe73 antibody, HIF-2alpha antibody, HRF antibody, Hif2a antibody, bhlhe73 antibody, ecyt4 antibody, hif2 antibody, hif2a antibody, hif2alpha antibody, hlf antibody, mop2 antibody, pasd2 antibody, EPAS antibody, EPAS-1 antibody, endothelial PAS domain protein 1 antibody, endothelial PAS domain protein 1 L homeolog antibody, EPAS1 antibody, epas1 antibody, Epas1 antibody, epas1.L antibody

Background: HIF-2 alpha is also designated EPAS1 whose gene is mapped to 2p21-p16. The predicted mouse protein is 88 % identical to human EPAS1. The human EPAS1 gene contains 15 exons and spans at least 120 kb. The positions of the introns within the genomic region encoding the N-terminal bHLH-PAS domains of EPAS1 and AHR are similar, suggesting that the 5-prime ends of the 2 genes may have arisen from a gene duplication event1. Moreover, the predicted protein shares 48 % sequence identity with HIF1-alpha, a bHLH-PAS transcription factor that induces EPO gene expression in cultured cells in response to hypoxia. Like HIF1A, EPAS1 binds to and activates transcription from the HIF1A response element derived from the 3-prime flanking region of the EPO gene. EPAS1 is predominantly expressed in highly vascularized tissues of adult humans and in endothelial cells of the mouse adult and embryo. Furthermore, EPAS1 may represent an important regulator of vascularization, perhaps involving the regulation of endothelial cell gene expression in response to hypoxia2. HIF2A is expressed at relatively higher levels in villus sections of placenta and in lung samples compared with other tissues examined3. In addition, The variation in EPAS1 influences the relative contribution of aerobic and anaerobic metabolism and hence the maximum sustainable metabolic power for a given event duration4.

Synonyms: Basic helix loop helix PAS protein MOP2 antibody|Basic-helix-loop-helix-PAS protein MOP2 antibody|bHLHe73 antibody|Class E basic helix-loop-helix protein 73 antibody|ECYT4 antibody|Endothelial PAS domain containing protein 1 antibody|Endothelial pas domain protein 1 antibody|Endothelial PAS domain-containing protein 1 antibody|EPAS 1 antibody|EPAS-1 antibody|EPAS1 antibody|EPAS1_HUMAN antibody|HIF 1 alpha like factor antibody|HIF 2 alpha antibody|HIF-1-alpha-like factor antibody|HIF-2-alpha antibody|HIF2-alpha antibody|Hif2a antibody|HLF antibody|Hypoxia inducible factor 2 alpha antibody|Hypoxia inducible factor 2 alpha subunit antibody|Hypoxia-inducible factor 2-alpha antibody|Member of PAS protein 2 antibody|Member of pas superfamily 2 antibody|MOP 2 antibody|MOP2 antibody|PAS domain-containing protein 2 antibody|PASD2 antibody

UniProt: Q99814

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect