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CD3 antibody

CD3 Reactivity: Rat FACS, IP, IHC, IHC (fro), IHC (p) Host: Mouse Monoclonal 1F4 unconjugated

Catalog No. ABIN305267

Datasheet as PDF

Target See all CD3 Antibodies

CD3
Reactivity
181

90

22

15

15

10

8

8

7

5

3

3

2

1
Rat
Host
206

45

14

13

9
Mouse
Compatible Secondaries
Clonality
276

7
Monoclonal
Conjugate
85

32

27

22

21

11

7

6

6

5

4

3

3

3

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD3 antibody is un-conjugated
Application
259

79

53

44

42

35

28

23

9

8

7

7

4

3

2

2

2

2

2

2

2

1
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity

Recognizes rat CD3, a 25kD antigen which is found on rat T-cells. Does not react with rat B cells. In immunohistology it stains rat thymus tissues strongly in the medulla and weakly in the cortex. Functionally the addition of the antibody to a culture of rat T cells induces the proliferation of T-cells in the presence of PMA.

Purification

Ammonium sulfate precipitation

Immunogen

F344 rat T cells stimulated with PMA (TPA) and calcium ionophore.

Type of Immunogen: Cells

Clone

1F4

Isotype

IgM

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CD3 Reactivity: Human FACS, IP, CyTOF Host: Mouse Monoclonal MEM-57 unconjugated

anti-CD3 (CD3) antibody
CD3 Reactivity: Human FACS, IP, Func, CyTOF Host: Mouse Monoclonal MEM-57 unconjugated

anti-CD3 (CD3) antibody
CD3 Reactivity: Human, Non-Human Primate FACS, IP, IHC (fro), Func, CyTOF Host: Mouse Monoclonal UCHT1 unconjugated

anti-CD3 (CD3) antibody (Biotin)
CD3 Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-57 Biotin

anti-CD3 (CD3) antibody (Biotin)
CD3 Reactivity: Human, Non-Human Primate FACS, IP, IHC (fro) Host: Mouse Monoclonal UCHT1 Biotin

anti-CD3 (CD3) antibody (APC)
CD3 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal UCHT1 APC

anti-CD3 (CD3) antibody (PerCP)
CD3 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal UCHT1 PerCP

anti-CD3 (CD3) antibody (PE)
CD3 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal UCHT1 PE

Application Notes

Approved: Flo (1:10 - 1:25), IHC, IHC-Fr (1:10 - 1:25), IHC-P (1:10), IP

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request.

Comment

Target Species of Antibody: Rat

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

PBS, 0.09 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Target

CD3

Alternative Name

CD3 (CD3 Products)

Synonyms

CD3 antibody, AI504783 antibody, CD3epsilon antibody, T3e antibody, cd3-gamma antibody, cd3g antibody, cd3g-a antibody, cd3g/d antibody, cd3gd antibody, t3g antibody, CD3e molecule antibody, CD3d molecule antibody, CD3 antigen, epsilon polypeptide antibody, CD3g molecule S homeolog antibody, CD3E antibody, CD3D antibody, Cd3e antibody, cd3g.S antibody

Pathways

TCR Signaling, Ubiquitin Proteasome Pathway