ERBB3 antibody (AA 1330-1342)

Catalog No. ABIN305733

The Goat Polyclonal anti-ERBB3 antibody (ABIN305733) specifically detects ERBB3 in WB, IHC, ELISA and IHC (p). The antibody is reactive with Human, Rat, Monkey, Horse, Pig, Bat and Hamster samples.

Quick Overview for ERBB3 antibody (AA 1330-1342) (ABIN305733)

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Reactivity: Human, Rat, Monkey, Horse, Pig, Bat, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This ERBB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ERBB3 Antibody

Binding Specificity: AA 1330-1342

Specificity: Human ERBB3. This antibody is expected to recognise isoform 1 (NP_001973.2) only.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Rat, Bat, Hamster, Panda, Horse, Pig (100%) Mouse, Bovine (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-HSRLFPKANAQRT from the C-terminus of human ERBB3 (NP_001973.2). Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Rat, Bat, Hamster, Panda, Horse, Pig (100%), Mouse, Bovine (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (0.1 - 0.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ERBB3

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Alternative Name: HER3 / ERBB3

Background: Name/Gene ID: ERBB3
Subfamily: EGF Receptor
Family: Protein Kinase

Synonyms: ERBB3, C-erbB-3, C-erbB3, ErbB-3, HER3, p85-sErbB3, p45-sErbB3, MDA-BF-1, ErbB3-S, LCCS2, p180-ErbB3

Gene ID: 2065

NCBI Accession: NP_001973

UniProt: P21860

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway