SPARC antibody

Catalog No. ABIN307028

Product Details anti-SPARC Antibody

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPARC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Cross-Reactivity: Human

Characteristics: Secreted Protein Acidic and Rich in Cysteine, SPARC, SPARC-related modular calcium-binding protein 2 [Precursor], Secreted modular calcium-binding protein 2, SMOC-2, Smooth muscle-associated protein 2, SMAP-2,SPARC is a key factor in cell-matrix interactions and possibly tumour aggressiveness. The SPARC gene, which encodes a multifunctional glycoprotein with roles in tissue development, remodelling and fibrosis. A regulator of cell-extracellular matrix (ECM) interactions, SPARC represents a major factor in the ECM remodelling occurring during tumour invasion. in silico analysis reveals 4 UTR-SNPs located in the 3 -UTR of the SPARC gene, corresponding to 1474 g a, 1551 g c, 1922 t g and 2072 c t changes, which are significantly associated with tumoral state of the tissue. Of all hits, the 2072 SPARC polymorphism had the best association with cancer. SPARC therefore is a gene involved in a number of diseases including rheumatoid arthritis, scleroderma, tumor development and metastasis. SPARC variants have been detected in tumour samples of patients with acute myeloblastic leukemia (AML).

Purification: Ammonium Sulfate Precipitation

Immunogen: Rabbit immunized with a synthetic peptide derived from the human SPARC protein.

Isotype: IgG

Application Details

Application Notes: Antibody can be used for Western blotting (1-5 μg/mL), Immunohistochemistry and ELISA. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Alternative Name: SPARC (SPARC Products)

Synonyms: ON antibody, AA517111 antibody, BM-40 antibody, hm:zeh0062 antibody, SPARC antibody, sparc antibody, secreted protein acidic and cysteine rich antibody, secreted acidic cysteine rich glycoprotein antibody, secreted protein, acidic, cysteine-rich (osteonectin) antibody, secreted protein, acidic, cysteine-rich (osteonectin) L homeolog antibody, secreted protein, acidic, cysteine-rich (osteonectin) S homeolog antibody, SPARC antibody, Sparc antibody, sparc antibody, sparc.L antibody, sparc.S antibody

UniProt: P09486

Pathways: Autophagy