GLUT1 antibody (C-Term)

Catalog No. ABIN307185

The Rabbit Polyclonal anti-GLUT1 antibody (ABIN307185) specifically detects GLUT1 in WB, IHC, IF and IHC (p). The antibody is reactive with Human and Rat samples.

Quick Overview for GLUT1 antibody (C-Term) (ABIN307185)

Target: GLUT1 (SLC2A1) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 1 (SLC2A1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLUT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GLUT1 Antibody

Binding Specificity: C-Term

Specificity: Recognizes human Glut-1.

Purification: Immunoaffinity purified

Immunogen: A synthetic peptide derived from C-terminal of human GLUT-1Cellular Localization: Membrane and focal cytoplasmic.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IF, IHC, IHC-P (5 μg/mL), WB (2 - 4 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 2-4 μg/mL for 2hrs. Positive control: Rat brain lysate, breast carcinoma, squamous cell carcinoma, non-small-cell lung carcinoma, esophageal carcinoma.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6, 0.2 % BSA, 15 mM sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GLUT1

Target: GLUT1 (SLC2A1) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 1 (SLC2A1))

Alternative Name: SLC2A1 / GLUT-1

Background: Name/Gene ID: SLC2A1
Subfamily: Sugar transporter
Family: Transporter

Synonyms: SLC2A1, EIG12, GLUT1DS, DYT17, DYT18, DYT9, Glucose transporter 1, HepG2 glucose transporter, Glucose transporter GLUT1, GLUT-1, GLUT1, PED

Gene ID: 6513

UniProt: P11166

Pathways: Sensory Perception of Sound, Dicarboxylic Acid Transport, Warburg Effect