GLUT1 antibody (AA 203-305)

Catalog No. ABIN6940594

The Mouse Monoclonal anti-GLUT1 antibody (Clone GLUT1-2475) (ABIN6940594) specifically detects GLUT1 in WB, IHC, FACS, IF, ELISA, Coat and StM. The antibody is reactive with Human samples.

Quick Overview for GLUT1 antibody (AA 203-305) (ABIN6940594)

Target: GLUT1 (SLC2A1) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 1 (SLC2A1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GLUT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA, Coating (Coat), Staining Methods (StM)

Clone: GLUT1-2475

Product Details anti-GLUT1 Antibody

Binding Specificity: AA 203-305

Specificity: Recognizes a protein of 55 kDa, which is identified as GLUT-1. Glucose transporters are integral membrane glycoproteins involved in transporting glucose into most cells. There are many types of glucose transport carrier proteins, designated as Glut-1 to Glut-12. Glut-1 is a major glucose transporter in the mammalian blood-brain barrier. It is expressed in high density on the membranes of human erythrocytes and the brain capillaries that comprise the blood-brain barrier. Glut-1 is expressed at variable levels in many human tissues. Overexpression of Glut-1 has been linked to tumor progression or poor survival of patients with carcinomas of the colon, breast, cervical, lung, bladder and mesothelioma. Glut-1 is a sensitive and specific marker for the differentiation of malignant mesothelioma (positive) from reactive mesothelium (negative).

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment of human GLUT1 protein (around aa 203-305) (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: K562, A431, MDA-MB-231 cells. Erythrocytes. Mesothelioma or breast, colon and ovarian carcinoma.

Known Application: ELISA (For coating use Ab at 1-2 μg/mL order Ab without BSA), Flow Cytometry (1-2 μg/million cells),Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for GLUT1

Target: GLUT1 (SLC2A1) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 1 (SLC2A1))

Alternative Name: SLC2A1

Molecular Weight: 55kDa

Gene ID: 6513

UniProt: P11166

Pathways: Sensory Perception of Sound, Dicarboxylic Acid Transport, Warburg Effect