GLUT1 antibody (AA 92-492)

Catalog No. ABIN3043927

This Rabbit Polyclonal antibody specifically detects GLUT1 in WB, IHC, FACS, IF, ICC and IHC (fro). It exhibits reactivity toward Human, Rat and Mouse.It has been mentioned in 1 publication

Quick Overview for GLUT1 antibody (AA 92-492) (ABIN3043927)

Target: GLUT1 (SLC2A1) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 1 (SLC2A1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLUT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GLUT1 Antibody

Binding Specificity: AA 92-492

Purpose: Anti-Glucose Transporter GLUT1/SLC2A1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Glucose Transporter GLUT1/SLC2A1 Antibody (ABIN3043927). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SLC2A1 recombinant protein (Position: R92-V492). Human SLC2A1 shares 98% and 98.3% amino acid (aa) sequence identity with mouse and rat SLC2A1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human, -
Immunocytochemistry/Immunofluorescence , 2 μg/mL, Human, -
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) , IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-GLUT1 antibody (ABIN3043927)

Li, Zhao, Qi, Wang, Zhang, Li, Qin: "lncRNA Ftx promotes aerobic glycolysis and tumor progression through the PPARγ pathway in hepatocellular carcinoma." in: International journal of oncology, Vol. 53, Issue 2, pp. 551-566, (2018) (PubMed).

Target Details for GLUT1

Target: GLUT1 (SLC2A1) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 1 (SLC2A1))

Alternative Name: SLC2A1

Background:

Synonyms: Solute carrier family 2, facilitated glucose transporter member 1,Glucose transporter type 1, erythrocyte/brain,GLUT-1,HepG2 glucose transporter,SLC2A1,GLUT1,

Tissue Specificity: Detected in erythrocytes (at protein level). Expressed at variable levels in many human tissues. .

Background: GLUT1, also known as SLC2A1, is a major glucose transporter in the mammalian blood-brain barrier whose gene is mapped to 1p35-p31.3 and contains 10 exons. It is present at high levels in primate erythrocytes and brain endothelial cells. Not only can transport dehydroascorbic acid (the oxidized form of vitamin C) into the brain, GLUT1 is also likely to contribute to HTLV-associated disorders through interacting with HTLV envelope glycoproteins. Functionally, GLUT1 deficiency causes a decrease in embryonic glucose uptake and apoptosis, which may be involved in diabetic embryopathy, by contrast, an increased expression of GLUT1 in some malignant tumors may suggest a role for glucose-derivative tracers to detect in vivo thyroid cancer metastases by positron-emission tomography scanning.

Sequence Similarities: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.

Molecular Weight: 55 kDa

Gene ID: 6513

UniProt: P11166

Pathways: Sensory Perception of Sound, Dicarboxylic Acid Transport, Warburg Effect