Pirin (PIR) (C-Term) antibody

Details for Product No. ABIN309704
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Antigen
Synonyms 2310042L19Rik, Pirnl, zgc:92778, ATPIRIN1, PRN1, pirin, xpir
Epitope
C-Term
(4), (4), (3), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine), Xenopus laevis, Zebrafish (Danio rerio), Human
(26), (4), (3), (1), (1)
Host
Rabbit
(10), (8), (7), (1)
Clonality
Polyclonal
Application
Western Blotting (WB)
(23), (10), (4), (4), (3), (3), (2), (2), (1), (1), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN309704
229.00 $
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Immunogen Synthetic peptide directed towards the C terminal of human PIR
Sequence EPHHTAVLGE GDSVQVENKD PKRSHFVLIA GEPLREPVIQ HGPFVMNTNE
Predicted Reactivity Human : 100 %, Dog : 90 %, Mouse : 90 %, Rat : 90 %, Bovine : 80 %, African clawed frog : 70 %, Zebrafish : 70 %
Characteristics This is a rabbit polyclonal antibody against PIR. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Alternative Name PIR
Background PIR is a member of the cupin superfamily. The protein is an Fe(II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication.This gene encodes a member of the cupin superfamily. The encoded protein is an Fe(II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Alternatively spliced transcript variants have been described.
Molecular Weight 32 kDa
Gene ID 8544
NCBI Accession NM_003662, NP_003653
UniProt O00625
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 290 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Pirin (PIR) (C-Term) antibody WB Suggested Anti-PIR Antibody Titration: 1.25ug/ml
Positive Control: HepG2 cell lysate
Background publications Adams, Jia: "Structural and biochemical analysis reveal pirins to possess quercetinase activity." in: The Journal of biological chemistry, Vol. 280, Issue 31, pp. 28675-82, 2005 (PubMed).

Validation Images
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