Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3) (C-Term) antibody

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Antigen
Synonyms UBE2L3, E2-F1, L-UBC, UBCH7, UbcM4, C79827, Ubce7, ube2l3l, wu:fb38h03
Epitope
C-Term
(41), (19), (17), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(101), (36), (31), (7), (5), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Host
Rabbit
(79), (17), (6)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (6), (6), (5), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(88), (44), (24), (16), (14), (11), (7), (6), (5), (3), (1), (1), (1), (1)
Pubmed 1 reference available
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Quantity 50 μg
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Immunogen Synthetic peptide directed towards the C terminal of human UBE2L3
Sequence IQSLIALVND PQPEHPLRAD LAEEYSKDRK KFCKNAEEFT KKYGEKRPVD
Predicted Reactivity African clawed frog : 100 %, Chicken : 100 %, Dog : 100 %, Guinea pig : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %, Zebrafish : 90 %
Characteristics This is a rabbit polyclonal antibody against UBE2L3. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name UBE2L3 (UBE2L3 Antibody Abstract)
Background UBE2L3(ubiquitin-conjugating enzyme E2L 3) Antibody The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Molecular Weight 17 kDa
Gene ID 7332
NCBI Accession NP_003338, NM_003347
UniProt P68036
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 154 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3) (C-Term) antibody (ABIN310414) WB Suggested Anti-UBE2L3 Antibody Titration: 0.2-1 ug/ml
Positive Contro...
Western Blotting (WB) image for anti-Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3) (C-Term) antibody (ABIN310414) WB Suggested Anti-UBE2L3
Antibody Titration: 0.2-1 µg/mL
Positive Contr...
Product cited in: Collins, Wright, Edwards et al.: "A genome annotation-driven approach to cloning the human ORFeome." in: Genome biology, Vol. 5, Issue 10, pp. R84, 2004 (PubMed).

Catalog No. ABIN310414
$289.00
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