Ring Finger Protein 40, E3 Ubiquitin Protein Ligase (RNF40) antibody

Antigen: Ring Finger Protein 40, E3 Ubiquitin Protein Ligase (RNF40)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Dog (Canine)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN310439

Additional Information

Alternative name: RNF40

Gene ID: RNF40

UniProt: O75150

Immunogen: The immunogen for anti-RNF40 antibody: synthetic peptide directed towards the C terminal of human RNF40

Sequence: KLLREEKDELGEQVLGLKSQVDAQLLTVQKLEEKERALQG SLGGVEKELT

Cross-Reactivity: Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)

Format: Lyophilized

Reconstitution: Add 100 µl of distilled water. Final anti-RNF40 antibody concentration is 1 mg/ml.

Description: RNF40 contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery.The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery.

Characteristics: Antigen Size: 1001 amino acids

Molecular Weight: 114kDa

Application Details

Application Notes: WB Suggested Anti-RNF40 Antibody Titration: 1.25ug/ml
Positive Control: 293T cell lysate.

Purification: Protein A purified

Buffer: PBS

Storage (Long Term): For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Storage (Short Term): Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.

Research Area: Proteolysis / Ubiquitin, Ion Channels

Restrictions: For Research Use only

Images

WB Suggested Anti-RNF40 Antibody Titration: 1.25ug/ml
Positive Control: 293T cell lysate

anti-Ring Finger Protein 40, E3 Ubiquitin Protein Ligase (RNF40) antibody (Image 2)

Publications

Product: Zhu, Zheng, Pham et al.: "Monoubiquitination of human histone H2B: the factors involved and their roles in HOX gene regulation." in: Molecular cell, Vol. 20, Issue 4, pp. 601-11, 2005 (PubMed).