Ring Finger Protein 128 (RNF128) (C-Term) antibody

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Antigen
Epitope
C-Term
(16), (13), (11), (8), (2), (1)
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(30), (14), (14), (1), (1)
Host
Rabbit
(43)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(31), (15), (11), (4), (1)
Pubmed 1 reference available
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Quantity 100 μg
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Immunogen Synthetic peptide directed towards the C terminal of human RNF128
Sequence LEEHVQSTNE SLQLVNHEAN SVAVDVIPHV DNPTFEEDET PNQETAVREI
Predicted Reactivity Human : 100 %, Dog : 90 %, Mouse : 90 %, Rat : 90 %
Characteristics This is a rabbit polyclonal antibody against RNF128. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Alternative Name RNF128 (RNF128 Antibody Abstract)
Background RNF128 is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of RNF128 in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of RNF128 was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype.The protein encoded by this gene is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of this gene in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of this gene was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Molecular Weight 47 kDa
Gene ID 79589
NCBI Accession NM_194463, NP_919445
UniProt Q8TEB7
Research Area Proteolysis / Ubiquitin
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 428 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-Ring Finger Protein 128 (RNF128) (C-Term) antibody (ABIN310450) WB Suggested Anti-RNF128 Antibody Titration: 1.25ug/ml
Positive Control:...
Western Blotting (WB) image for anti-Ring Finger Protein 128 (RNF128) (C-Term) antibody (ABIN310450) WB Suggested Anti-RNF128
Antibody Titration: 1.25 µg/mL
Positive Contro...
Background publications Soares, Seroogy, Skrenta et al.: "Two isoforms of otubain 1 regulate T cell anergy via GRAIL." in: Nature immunology, Vol. 5, Issue 1, pp. 45-54, 2003 (PubMed).