Polymerase (RNA) III (DNA Directed) Polypeptide B (POLR3B) (C-Term) antibody

Details for Product No. ABIN311087
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Antigen
Synonyms RGD1565311, C128, HLD8, RPC2, 2700078H01Rik, A330032P03Rik, C85372
Epitope
C-Term
(7), (7), (6), (2), (2), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Fruit Fly (Drosophila melanogaster), Dog (Canine), Human, Chicken
(17), (16), (9), (1)
Host
Rabbit
(23), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(23), (18), (3), (2), (2), (1)
Pubmed 1 reference available
Quantity 50 µg
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Catalog No. ABIN311087
289.00 $
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Immunogen Synthetic peptide directed towards the C terminal of human POLR3B
Sequence IEKVMISSNA EDAFLIKMLL RQTRRPEIGD KFSSRHGQKG VCGLIVPQED
Predicted Reactivity Bovine : 100 %, Chicken : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %, Zebrafish : 100 %
Characteristics This is a rabbit polyclonal antibody against POLR3B. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name POLR3B
Background POLR3B belongs to the RNA polymerase beta chain family. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3B is the second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. It is proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft.
Molecular Weight 128 kDa
Gene ID 55703
NCBI Accession NP_060552, NM_018082
UniProt Q9NW08
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 1133 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Product cited in: Yee, Gong, Huang et al.: "Mutation of RNA Pol III subunit rpc2/polr3b Leads to Deficiency of Subunit Rpc11 and disrupts zebrafish digestive development." in: PLoS biology, Vol. 5, Issue 11, pp. e312, 2007 (PubMed).

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