POLR3B antibody (AA 866-915)

Catalog No. ABIN6738508

Product Details anti-POLR3B Antibody

Target: POLR3B (Polymerase (RNA) III (DNA Directed) Polypeptide B (POLR3B))

Binding Specificity: AA 866-915

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Zebrafish (Danio rerio), Pig, Horse, Monkey, Guinea Pig, Drosophila melanogaster, Hamster, Xenopus laevis, Bat, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR3B antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human POLR3B

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa866-915 of human POLR3B (Q9NW08, NP_060552). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Salmon, Stickleback, Zebrafish, Drosophila, Beetle (100%), Sea squirt, Slime mold, Rice, Arabidopsis (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for POLR3B

Target: POLR3B (Polymerase (RNA) III (DNA Directed) Polypeptide B (POLR3B))

Alternative Name: POLR3B (POLR3B Products)

Synonyms: RGD1565311 antibody, C128 antibody, HLD8 antibody, RPC2 antibody, 2700078H01Rik antibody, A330032P03Rik antibody, C85372 antibody, RNA polymerase III subunit B antibody, polymerase (RNA) III (DNA directed) polypeptide B antibody, Polr3b antibody, POLR3B antibody

Background: Name/Gene ID: POLR3B

Synonyms: POLR3B, HLD8, RPC2, C128, RNA polymerase III subunit C2

Gene ID: 55703

NCBI Accession: NP_060552