DnaJ (Hsp40) Homolog, Subfamily C, Member 24 (DNAJC24) (N-Term) antibody

Details for Product No. ABIN311574
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Antigen
Synonyms DPH4, JJJ3, ZCSL3, 1700030A21Rik, 2610027M02Rik, AV066965, AW240712, Dph4, MmDjC7, Zcsl3, RGD1564710
Epitope
N-Term
(8), (7), (3), (2), (2), (1)
Reactivity
Mouse (Murine), Cow (Bovine), Human
(17), (3), (3)
Host
Rabbit
(17)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(17), (11)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN311574
289.00 $
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Immunogen Synthetic peptide directed towards the N terminal of human DPH4
Sequence MMAVEQMPKK DWYSILGADP SANISDLKQK YQKLILMYHP DKQSTDVPAG
Predicted Reactivity Human :100 %,   Mouse :90 %,   Bovine :80 %,  
Characteristics This is a rabbit polyclonal antibody against DPH4. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name DPH4
Background Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2, MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH4 is 1 of several enzymes involved in synthesis of diphthamide in EEF2.Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2, MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH4 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).
Molecular Weight 17 kDa
Gene ID 120526
NCBI Accession NP_859057, NM_181706
UniProt Q6P3W2
Research Area Signaling, Metabolism, Heat Shock Proteins
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 149 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-DnaJ (Hsp40) Homolog, Subfamily C, Member 24 (DNAJC24) (N-Term) antibody WB Suggested Anti-DPH4 Antibody Titration: 0.2-1 ug/ml
Positive Control: Human Liver
Background publications Liu, Milne, Kuremsky et al.: "Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2." in: Molecular and cellular biology, Vol. 24, Issue 21, pp. 9487-97, 2004 (PubMed).

Validation Images
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