CD8 antibody
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- Target See all CD8 Antibodies
- CD8
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD8 antibody is un-conjugated
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Application
- Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- This antibody recognizes the Human CD8 cell surface antigen, a 32 kDa glycoprotein expressed by the cytotoxic/suppressor subset of T-cells and weakly by NK cells. Clone 4B11 has been reported as being suitable for use in Western blotting. AM05530SU-N is recommended for this purpose.
- Characteristics
- Synonyms: T-lymphocyte differentiation antigen T8/Leu-2, T-cell surface glycoprotein CD8 alpha chain
- Immunogen
- Synthetic peptide derived from CD8 alpha chain sequence.
- Clone
- 4B11
- Isotype
- IgG2b
- Top Product
- Discover our top product CD8 Primary Antibody
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- Application Notes
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Immunohistochemistry on Frozen Sections: 1/20-1/40. Immunohistochemistry on paraffin sections: 1/20-1/40. This product requires antigenretrieval using heat treatment prior to staining of paraffin sections.1 mM EDTA pH 8.0 isrecommended for this purpose. Positive Control Tissue: Human Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
- Restore with 1 mL distilled water.
- Storage
- 4 °C/-20 °C
- Storage Comment
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Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one monthor (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - Expiry Date
- 12 months
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- Target
- CD8
- Alternative Name
- CD8 (CD8 Products)
- Synonyms
- CD8 antibody, Leu2 antibody, MAL antibody, p32 antibody, CD8a molecule antibody, CD8A antibody
- Background
- The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
- Gene ID
- 925
- UniProt
- P01732
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