This antibody detects an epitope located at the C-terminal (amino acids 26-32) of the brain natriuretic peptide (BNP), a secreted protein which is a member of the natriuretic peptide family. This antibody also detects proBNP.
Characteristics
Synonyms: NPPB
Purification
Purified IgG prepared by affinity chromatography on Protein A
NPPB
Reactivity: Human, Rat
IHC, WB, IP
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA. Western Blot: detects a band of approximately 4 kDa when using synthetic BNP and a bandof 14 kDa when using recombinant proBNP. Immunoassay. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Caution: (A full Health and Safety assessment is available upon request) This product containsSodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by
BNP1 antibody, NPPB antibody, BNP antibody, Bnf antibody, AA408272 antibody, natriuretic peptide B antibody, natriuretic peptide type B antibody, NPPB antibody, Nppb antibody
Background
BNP is a cardiac hormone, which is synthesised as a pro-hormone (proBNP), and is proteolytically cleaved to release a biologically active fragment (BNP), and an inactive fragment (NT-proBNP) into the circulation. BNP is predominantly secreted from the cardiac ventricles in response to volume and pressure overload, and results in a number of biological activities including natriuresis, diuresis, vasorelaxation, and inhibition of the sympathetic nervous system. A high concentration of BNP in the bloodstream is indicative of heart failure.Synonyms: NPPB