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CD8 antibody (FITC)

CD8 Reactivity: Mouse FACS Host: Rat Monoclonal 07-06-53 FITC

Catalog No. ABIN317090

Datasheet as PDF

Target See all CD8 Antibodies

CD8
Reactivity
194

56

26

12

12

11

9

9

9

9

8

7

7

6

6

6

5

5

5

3

3

2

2
Mouse
Host
213

39

36
Rat
Compatible Secondaries
Clonality
250

30

2
Monoclonal
Conjugate
91

38

30

22

11

8

6

6

5

5

5

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD8 antibody is conjugated to FITC
Application
253

63

47

44

42

42

28

19

17

14

14

14

8

5

4

3

1

1

1

1

1

1
Flow Cytometry (FACS)

Specificity

This antibody detects CD8a, the alpha chain of the CD8 antigen. CD8 is expressed on T-cells, and exists either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. This antibody Clone 53-6.7 has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells.

Characteristics

Synonyms: T-lymphocyte differentiation antigen T8/Leu-2, T-cell surface glycoprotein CD8 alpha chain

Purification

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen

Mouse thymus or spleen.

Clone

07-06-53

Isotype

IgG2a

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anti-CD8 (CD8) antibody (PE)
CD8 Reactivity: Human FACS Host: Mouse Monoclonal MEM-31 PE

anti-CD8 (CD8) (AA 22-187) antibody
CD8 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-CD8 (CD8) antibody
CD8 Reactivity: Human FACS, IP, CyTOF Host: Mouse Monoclonal MEM-31 unconjugated

anti-CD8 (CD8) antibody (PE)
CD8 Reactivity: Cat FACS Host: Mouse Monoclonal fCD8 PE

anti-CD8 (CD8) antibody
CD8 Reactivity: Human IHC, IP, WB, ELISA Host: Rabbit Monoclonal 3C3 unconjugated Recombinant Antibody

anti-CD8 (CD8) antibody (Biotin)
CD8 Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-31 Biotin

anti-CD8 (CD8) antibody (FITC)
CD8 Reactivity: Human FACS Host: Mouse Monoclonal MEM-31 FITC

anti-CD8 (CD8) antibody (PerCP)
CD8 Reactivity: Human FACS Host: Mouse Monoclonal MEM-31 PerCP

anti-CD8 (CD8) antibody (APC)
CD8 Reactivity: Human FACS Host: Mouse Monoclonal MEM-31 APC

Application Notes

Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

0.5mg/mL

Buffer

PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C/-20 °C

Storage Comment

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.

Expiry Date

12 months
Target

CD8

Alternative Name

CD8 (CD8 Products)

Synonyms

CD8 antibody, Leu2 antibody, MAL antibody, p32 antibody, CD8a molecule antibody, CD8A antibody

Background

CD8 functions as a co-receptor with the T-cell receptor and mediates efficient cell to cell interactions within the immune system. The CD8 alpha chain specifically binds to class I MHC molecules.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

Gene ID

12525

UniProt

P01731