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CD8 antibody

CD8 Reactivity: Mouse FACS, IHC (fro), IP, IHC (p) Host: Rat Monoclonal 07-06-53 unconjugated
Catalog No. ABIN317092
  • Target See all CD8 Antibodies
    CD8
    Reactivity
    • 194
    • 55
    • 25
    • 12
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    • 11
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    Mouse
    Host
    • 212
    • 38
    • 36
    Rat
    Clonality
    • 248
    • 30
    • 2
    Monoclonal
    Conjugate
    • 90
    • 38
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    • 22
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    • 1
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    This CD8 antibody is un-conjugated
    Application
    • 251
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    Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Specificity
    This antibody detects CD8a, the alpha chain of the CD8 antigen. CD8 is expressed on T-cells, and exists either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. The CD8a antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells.
    Cross-Reactivity (Details)
    Species reactivity (tested):Mouse.
    Characteristics
    Synonyms: T-lymphocyte differentiation antigen T8/Leu-2, T-cell surface glycoprotein CD8 alpha chain
    Purification
    Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    Immunogen
    Mouse thymus or spleen.
    Clone
    07-06-53
    Isotype
    IgG2a
    Top Product
    Discover our top product CD8 Primary Antibody
  • Application Notes
    Flow Cytometry: Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing lowaffinity Fc receptors. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: This antibody has been reported as workingwell on Zinc-fixed paraffin-embedded sections. However, inconsistent results have beenreported on formalin-fixed paraffin-embedded tissue sections. Immunoprecipitation.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Phosphate buffered saline pH 7.2 containing 0.09 % Sodium Azide as preservative
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
    Shelf life: one year from despatch.
    Expiry Date
    12 months
  • Target
    CD8
    Alternative Name
    CD8 (CD8 Products)
    Synonyms
    CD8 antibody, Leu2 antibody, MAL antibody, p32 antibody, CD8a molecule antibody, CD8A antibody
    Background
    The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. CD8 is expressed on most thymocytes and approximately 1/3 of peripheral blood T cells in humans. CD8 alpha/beta heterodimers are expressed only on TCR alpha/beta T cells, whereas CD8 alpha homodimers can be expressed on alpha/beta and gamma/delta T cells and some NK cells in humans. The pattern of expression in other species has not been well documented.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
    Gene ID
    12525
    UniProt
    P01731
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